{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T16:29:31Z","timestamp":1785601771908,"version":"3.56.0"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T00:00:00Z","timestamp":1503619200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003725","name":"National Research Foundation of Korea","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003725","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Amoxicillin (Amx) is one of the most important antibiotics for eradication of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H.\u00a0pylori<\/jats:italic>). Main determinants of genetically stable Amx resistance are mutations in the C\u2010terminus of penicillin\u2010binding protein 1A (<jats:italic>pbp1A<\/jats:italic>). However, contribution of individual mutation remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>77 Amx\u2010resistant (Amx<jats:sup>R<\/jats:sup>) and 77 Amx\u2010susceptible (Amx<jats:sup>S<\/jats:sup>) <jats:italic>H.\u00a0pylori<\/jats:italic> strains were isolated from gastric tissues, and <jats:styled-content style=\"fixed-case\">DNA<\/jats:styled-content> sequencing was performed to compare C\u2010terminus sequences of <jats:italic>pbp1A<\/jats:italic> gene between Amx<jats:sup>R<\/jats:sup> and Amx<jats:sup>S<\/jats:sup> strains. Natural transformation of these mutated genes into amoxicillin\u2010susceptible strains was performed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Among many mutations in <jats:italic>pbp1A<\/jats:italic>, D479E (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>: 37.4, 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 5.53\u2010252.49, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.001), and T593 mutation (<jats:styled-content style=\"fixed-case\">OR<\/jats:styled-content>: 32.0, 95% <jats:styled-content style=\"fixed-case\">CI<\/jats:styled-content>: 4.04\u2010252.86, <jats:italic>P\u00a0<\/jats:italic>&lt;\u00a0.001) independently contributed to Amx resistance in <jats:italic>H.\u00a0pylori<\/jats:italic> strains. In the transformation experiment, T593 mutations were identified in their transformants showing Amx resistance. However, <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> product of D479E was not inserted into recipient (<jats:styled-content style=\"fixed-case\">ATCC<\/jats:styled-content> 43504) resulting in transformation failure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Amx resistance is associated with various substitutions in <jats:italic>pbp1A<\/jats:italic> and T593 mutation contributes to Amx resistance of <jats:italic>H.\u00a0pylori<\/jats:italic>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12437","type":"journal-article","created":{"date-parts":[[2017,8,25]],"date-time":"2017-08-25T12:06:37Z","timestamp":1503662797000},"source":"Crossref","is-referenced-by-count":19,"title":["Specific mutations of penicillin\u2010binding protein 1A in 77 clinically acquired amoxicillin\u2010resistant <i>Helicobacter pylori<\/i> strains in comparison with 77 amoxicillin\u2010susceptible strains"],"prefix":"10.1111","volume":"22","author":[{"given":"Yong Hwan","family":"Kwon","sequence":"first","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam South Korea"},{"name":"Department of Internal Medicine Kyungpook National University Hospital  Daegu South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Yeon","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Liver Research Institute Seoul National University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9397-0406","authenticated-orcid":false,"given":"Nayoung","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam South Korea"},{"name":"Department of Internal Medicine and Liver Research Institute Seoul National University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji Hyun","family":"Park","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine and Liver Research Institute Seoul National University College of Medicine  Seoul South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryoung Hee","family":"Nam","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sun Min","family":"Lee","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang Hospital  Seongnam South Korea"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jin\u2010Wook","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Internal Medicine Seoul National University Bundang 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