{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T09:56:00Z","timestamp":1791021360425,"version":"4.1.0"},"reference-count":32,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T00:00:00Z","timestamp":1508716800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2017,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>There are several methods for <jats:italic>Helicobacter pylori<\/jats:italic> infection diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aim<\/jats:title><jats:p>The efficacies of three methods for <jats:italic>H. pylori<\/jats:italic> identification directly from a biopsy were compared: histology, culture, and molecular GenoType\u00ae HelicoDR test.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials &amp; Methods<\/jats:title><jats:p>Eighty\u2010five triplicates of stomach antrum biopsies were obtained during gastroscopy procedures for culture, histology, and molecular assay. In addition, we performed molecular identification of genes encoding resistance to clarithromycin and fluoroquinolones.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The results have shown that the most specific method with the highest number of positive specimens was by molecular kit, compared to culture and histology (94.3%, 77.1%, and 71.4%, respectively). There was a higher rate of resistance mutations to clarithromycin than to fluoroquinolones (68.26% vs 20%). The most common mutations for clarithromycin and fluoroquinolones resistance were found in alleles A2143G and N87K, respectively. The highest rate of positive specimens was identified by the molecular.<\/jats:p><\/jats:sec><jats:sec><jats:title>Discussion<\/jats:title><jats:p>GenoType HelicoDR kit (94.3%), which has several advantages: direct identification, strain resistance characterization, mixture of genotypes detection, and no transport or storage limitations; thus, it is an excellent epidemiological screening tool. This work has demonstrated a lower resistance rate to fluoroquinolones; it is possible that in the investigated geographic area treatment with fluoroquinolones may be preferable to clarithromycin. GenoType\u00ae HelicoDR test eliminates the need for culture performance and susceptibility tests for several common antibiotic agents and enables optimal and specific antibiotic treatment adjustment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>We recommend a combination of PCR assay and bacterial culture for a quick method of screening and more efficient identification of <jats:italic>H. pylori<\/jats:italic> strains and resistance patterns.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12447","type":"journal-article","created":{"date-parts":[[2017,10,23]],"date-time":"2017-10-23T11:08:41Z","timestamp":1508756921000},"source":"Crossref","is-referenced-by-count":30,"title":["GenoType\u00ae Helico<scp>DR<\/scp> test in comparison with histology and culture for <i>Helicobacter pylori<\/i> detection and identification of resistance mutations to clarithromycin and fluoroquinolones"],"prefix":"10.1111","volume":"22","author":[{"given":"Nina","family":"Pastukh","sequence":"first","affiliation":[{"name":"Clinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine Bar Ilan University  Galilee Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dana","family":"Binyamin","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine Bar Ilan University  Galilee Israel"},{"name":"The Faculty of Medicine in the Galilee Bar Ilan University  Zefat Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Avi","family":"On","sequence":"additional","affiliation":[{"name":"The Faculty of Medicine in the Galilee Bar Ilan University  Zefat Israel"},{"name":"Pediatric Gastrointestinal Unit Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine Bar Ilan University  Galilee Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maya","family":"Paritsky","sequence":"additional","affiliation":[{"name":"The Faculty of Medicine in the Galilee Bar Ilan University  Zefat Israel"},{"name":"Gastrointestinal Unit Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine Bar Ilan University  Galilee Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9012-6015","authenticated-orcid":false,"given":"Avi","family":"Peretz","sequence":"additional","affiliation":[{"name":"Clinical Microbiology Laboratory Baruch Padeh Medical Center, Poriya, affiliated with the Faculty of Medicine Bar Ilan University  Galilee Israel"},{"name":"The Faculty of Medicine in the Galilee Bar Ilan University  Zefat Israel"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2017,10,23]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI20925"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2857"},{"key":"e_1_2_7_4_1","first-page":"409","article-title":"Worldwide H. pylori antibiotic resistance: a systematic review","volume":"19","author":"De Francesco V","year":"2010","journal-title":"J Gastrointestin Liver Dis"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1093\/jac\/dkp445"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v16.i18.2272"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1440-1746.2009.06220.x"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12071"},{"key":"e_1_2_7_9_1","doi-asserted-by":"publisher","DOI":"10.1097\/00042737-201204001-00004"},{"key":"e_1_2_7_10_1","doi-asserted-by":"publisher","DOI":"10.1186\/1472-6963-10-344"},{"key":"e_1_2_7_11_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2011.00875.x"},{"key":"e_1_2_7_12_1","doi-asserted-by":"publisher","DOI":"10.1586\/erm.11.17"},{"key":"e_1_2_7_13_1","unstructured":"BialekR ZelckCF KastlL.RIDA\u00aeGENE Helicobacter pylori is a sensitive and specific real\u2010time PCR test for the simultaneous detection and differentiation of Helicobacter pylori and its resistance to clarithromycin in human tissue biopsies. 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