{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T12:32:46Z","timestamp":1753878766966,"version":"3.41.2"},"reference-count":38,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T00:00:00Z","timestamp":1510012800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["16K08708"],"award-info":[{"award-number":["16K08708"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Non\u2010<jats:italic>Helicobacter pylori<\/jats:italic> helicobacters (<jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s) besides <jats:italic>H.\u00a0pylori<\/jats:italic> infect human stomachs and cause chronic gastritis and mucosa\u2010associated lymphoid tissue lymphoma. Cholesteryl\u2010\u03b1\u2010glucosides have been identified as unique glycolipids present in <jats:italic>H.\u00a0pylori<\/jats:italic> and some <jats:italic>Helicobacter<\/jats:italic> species. Cholesterol\u2010\u03b1\u2010glucosyltransferase (\u03b1CgT), a key enzyme for the biosynthesis of cholesteryl\u2010\u03b1\u2010glucosides, plays crucial roles in the pathogenicity of <jats:italic>H.\u00a0pylori<\/jats:italic>. Therefore, it is important to examine \u03b1CgTs of <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Six gastric <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s were isolated from Japanese patients and maintained in mouse stomachs. The \u03b1<jats:italic>CgT<\/jats:italic> genes were amplified by <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and inverse <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. We retrieved the \u03b1<jats:italic>CgT<\/jats:italic> genes of other <jats:italic>Helicobacter<\/jats:italic> species by <jats:styled-content style=\"fixed-case\">BLAST<\/jats:styled-content> searches in GenBank.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>\u03b1<jats:italic>CgT<\/jats:italic> genes were present in most <jats:italic>Helicobacter<\/jats:italic> species and in all Japanese isolates examined. However, we could find no candidate gene for \u03b1CgT in the whole genome of <jats:italic>Helicobacter cinaedi<\/jats:italic> and several enterohepatic species. Phylogenic analysis demonstrated that the \u03b1<jats:italic>CgT<\/jats:italic> genes of all Japanese isolates show high similarities to that of a zoonotic group of gastric <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s including <jats:italic>Helicobacter suis<\/jats:italic>,<jats:italic> Helicobacter heilmannii<\/jats:italic>, and <jats:italic>Helicobacter ailurogastricus<\/jats:italic>. Of 6 Japanese isolates, the \u03b1<jats:italic>CgT<\/jats:italic> genes of 4 isolates were identical to that of <jats:italic>H.\u00a0suis<\/jats:italic>, and that of another 2 isolates were similar to that of <jats:italic>H.\u00a0heilmannii<\/jats:italic> and <jats:italic>H.\u00a0ailurogastricus<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>All gastric <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s examined showed presence of \u03b1<jats:italic>CgT<\/jats:italic> genes, indicating that \u03b1CgT may be beneficial for these helicobacters to infect human and possibly animal stomachs. Our study indicated that <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s could be classified into 2 groups, <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s with \u03b1<jats:italic>CgT<\/jats:italic> genes and <jats:styled-content style=\"fixed-case\">NHPH<\/jats:styled-content>s without \u03b1<jats:italic>CgT<\/jats:italic> genes.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12449","type":"journal-article","created":{"date-parts":[[2017,11,7]],"date-time":"2017-11-07T08:38:48Z","timestamp":1510043928000},"source":"Crossref","is-referenced-by-count":9,"title":["<i>Cholesterol\u2010<\/i>\u03b1<i>\u2010glucosyltransferase<\/i> gene is present in most <i>Helicobacter<\/i> species including gastric non\u2010<i>Helicobacter pylori<\/i> helicobacters obtained from Japanese patients"],"prefix":"10.1111","volume":"23","author":[{"given":"Masatomo","family":"Kawakubo","sequence":"first","affiliation":[{"name":"Department of Molecular Pathology Shinshu 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