{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,24]],"date-time":"2026-01-24T01:51:03Z","timestamp":1769219463419,"version":"3.49.0"},"reference-count":0,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T00:00:00Z","timestamp":1511395200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,2]]},"abstract":"Abstract<\/jats:title>Introduction<\/jats:title>Antibiotic resistance is a major contributing factor in treatment failure of Helicobacter pylori<\/jats:italic> eradication. Rifabutin (RB<\/jats:styled-content>) is a rescue treatment and rifampicin (RP<\/jats:styled-content>) is used to screen RB<\/jats:styled-content> resistance in vitro. The aim of this study was to evaluate the rate of rifamycins resistance and to determine the mutations in the rpo<\/jats:italic>B gene conferring resistance to discuss the current break point.<\/jats:p><\/jats:sec>Methods<\/jats:title>Antimicrobial susceptibility to RP<\/jats:styled-content> was first determined by E<\/jats:italic>\u2010test for 1015 H.\u00a0pylori<\/jats:italic> isolates. RP<\/jats:styled-content> and RB MIC<\/jats:styled-content>s were then determined by agar dilution method for strains with MIC<\/jats:styled-content> of RP<\/jats:styled-content>\u00a0>\u00a01\u00a0mg\/L, and the rpo<\/jats:italic>B gene was sequenced.<\/jats:p><\/jats:sec>Results<\/jats:title>Overall, 54 of 1015 strains exhibited a RP MIC<\/jats:styled-content>\u00a0>\u00a01\u00a0mg\/L by agar dilution method. Among these 54 strains, 10 had MIC<\/jats:styled-content>s of RP<\/jats:styled-content>\u00a0>\u00a04\u00a0mg\/L and RB<\/jats:styled-content>\u00a0\u2265\u00a01\u00a0mg\/L. They all carried at least one mutation in the rpo<\/jats:italic>B gene at codons 530, 538, 540, 525 in the RP<\/jats:styled-content> resistance\u2010determining region (RRDR<\/jats:styled-content>). Implication of the mutation L547F was confirmed by site\u2010directed mutagenesis experiment. In contrast, among the 44 H.\u00a0pylori<\/jats:italic> isolates with a MIC<\/jats:styled-content> of RP<\/jats:styled-content> comprised between 2 and 4\u00a0mg\/L, only 4 of 44 (9%) strains exhibited a mutation in rpoB<\/jats:italic>, but outside RRDR<\/jats:styled-content> (codons 470, 499, 636, or 657). For 31 of 44 tested strains, the RB MIC<\/jats:styled-content>s were \u22640.064\u00a0mg\/L.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results suggest that H.\u00a0pylori<\/jats:italic> isolates should be classified as resistant to RP<\/jats:styled-content> for MIC<\/jats:styled-content>s\u00a0>\u00a04\u00a0mg\/L. We considered that the optimal cut off for RB<\/jats:styled-content> was \u22650.125\u00a0mg\/L. We report a new mutation responsible for rifamycins, resistance, L547F.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12451","type":"journal-article","created":{"date-parts":[[2017,11,23]],"date-time":"2017-11-23T10:46:44Z","timestamp":1511434004000},"source":"Crossref","is-referenced-by-count":40,"title":["Molecular characterization of Helicobacter pylori<\/i> resistance to rifamycins"],"prefix":"10.1111","volume":"23","author":[{"given":"Constantin","family":"Hays","sequence":"first","affiliation":[{"name":"Bacteriology University of Paris\u2010Descartes Cochin Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christophe","family":"Burucoa","sequence":"additional","affiliation":[{"name":"EA 4331 LITEC Universit\u00e9 de Poitiers Laboratoire de bact\u00e9riologie, Hygi\u00e8ne CHU de Poitiers Poitiers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Philippe","family":"Lehours","sequence":"additional","affiliation":[{"name":"INSERM UMR1053 Bordeaux Research in Translational Oncology BaRITOn Universit\u00e9 de Bordeaux Bordeaux France"},{"name":"French National Reference Centre for Campylobacters and Helicobacter Bordeaux Hospital Bordeaux France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Cong Tri","family":"Tran","sequence":"additional","affiliation":[{"name":"EA 4331 LITEC Universit\u00e9 de Poitiers Laboratoire de bact\u00e9riologie, Hygi\u00e8ne CHU de Poitiers Poitiers France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana\u00efs","family":"Leleu","sequence":"additional","affiliation":[{"name":"Bacteriology University of Paris\u2010Descartes Cochin Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4274-9795","authenticated-orcid":false,"given":"Josette","family":"Raymond","sequence":"additional","affiliation":[{"name":"Bacteriology University of Paris\u2010Descartes Cochin Hospital Paris France"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2017,11,23]]},"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhel.12451","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12451","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,29]],"date-time":"2023-09-29T05:39:06Z","timestamp":1695965946000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hel.12451"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2017,11,23]]},"references-count":0,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2018,2]]}},"alternative-id":["10.1111\/hel.12451"],"URL":"https:\/\/doi.org\/10.1111\/hel.12451","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2017,11,23]]},"article-number":"e12451"}}