{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,3]],"date-time":"2026-07-03T17:07:19Z","timestamp":1783098439195,"version":"3.54.6"},"reference-count":30,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T00:00:00Z","timestamp":1513123200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"Victorian Government's Operational Infrastructure Support Program"},{"name":"Australian Government Australian Postgraduate Award"},{"name":"National Health and Medical Research Council of Australia"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> has undergone considerable adaptation to allow chronic persistence within the gastric environment. While <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010associated diseases are driven by an excessive inflammation, severe gastritis is detrimental to colonization by this pathogen. Hence, <jats:italic>H.\u00a0pylori<\/jats:italic> has developed strategies to minimize the severity of gastritis it triggers in its host. Superoxide dismutase (<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>) is well known for its role in protecting against oxidative attack; less recognized is its ability to inhibit immunity, shown for <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> from mammalian sources and those of some bacterial species. This study examined whether <jats:italic>H.\u00a0pylori <\/jats:italic><jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> (Hp<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content>) has the ability to inhibit the host immune response to these bacteria.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The ability of recombinant Hp<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> to modify the response to <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> was measured using mouse macrophages. A monoclonal antibody against Hp<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> was generated and injected into <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010infected mice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Addition of Hp<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> to cultures of mouse macrophages significantly inhibited the pro\u2010inflammatory cytokine response to <jats:styled-content style=\"fixed-case\">LPS<\/jats:styled-content> stimulation. A monoclonal antibody was generated that was specific for <jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> from <jats:italic>H.\u00a0pylori<\/jats:italic>. When injected into mice infected with <jats:italic>H.\u00a0pylori<\/jats:italic> for 3\u00a0months, this antibody was readily detected in both sera and gastric tissues 5\u00a0days later. While treatment with anti\u2010Hp<jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> had no effect on <jats:italic>H.\u00a0pylori<\/jats:italic> colonization at this time point, it significantly increased the levels of a range of pro\u2010inflammatory cytokines in the gastric tissues. This did not occur with antibodies against other antioxidant enzymes.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">SOD<\/jats:styled-content> from <jats:italic>H.\u00a0pylori<\/jats:italic> can inhibit the production of pro\u2010inflammatory cytokine during in vivo infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12459","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T06:12:52Z","timestamp":1513145572000},"source":"Crossref","is-referenced-by-count":16,"title":["Superoxide dismutase from <i>Helicobacter pylori<\/i> suppresses the production of pro\u2010inflammatory cytokines during in vivo infection"],"prefix":"10.1111","volume":"23","author":[{"given":"Andrew","family":"Stent","sequence":"first","affiliation":[{"name":"School of Veterinary and Agricultural Science Centre for Animal Biotechnology University of Melbourne  Parkville Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alison L.","family":"Every","sequence":"additional","affiliation":[{"name":"School of Veterinary and Agricultural Science Centre for Animal Biotechnology University of Melbourne  Parkville Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yok T.","family":"Chionh","sequence":"additional","affiliation":[{"name":"School of Veterinary and Agricultural Science Centre for Animal Biotechnology University of Melbourne  Parkville Vic. Australia"},{"name":"Murdoch Children's Research Institute Royal Children's Hospital  Parkville Vic. Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Garrett Z.","family":"Ng","sequence":"additional","affiliation":[{"name":"School of Veterinary and Agricultural Science Centre for Animal Biotechnology University of Melbourne  Parkville Vic. 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