{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,11]],"date-time":"2025-11-11T13:24:02Z","timestamp":1762867442961,"version":"3.41.2"},"reference-count":25,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T00:00:00Z","timestamp":1528243200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100003107","name":"Bundesministerium f\u00fcr Gesundheit","doi-asserted-by":"publisher","award":["1369\u2010339"],"award-info":[{"award-number":["1369\u2010339"]}],"id":[{"id":"10.13039\/501100003107","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Antimicrobial susceptibility of <jats:italic>Helicobacter (H.) pylori<\/jats:italic> is usually determined by phenotypic methods. When <jats:italic>H.\u00a0pylori<\/jats:italic> cannot be grown owing to contaminations or delay in transport of gastric tissue samples to the microbiological laboratory, molecular genetic testing is a reasonable alternative. The aim of this retrospective study was to assess the outcome of salvage eradication treatments based on molecular genetic susceptibility testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Data on 144 <jats:italic>H.\u00a0pylori<\/jats:italic> PCR\u2010positive gastric tissue samples of patients primarily with prior unsuccessful eradication treatments were retrospectively analyzed. Eradication treatments were recommended based on genotypic clarithromycin and\/or levofloxacin susceptibility as tested by real\u2010time PCR or reverse hybridization. Treatment success was assessed by attending physicians using urea breath test; stool\u2010antigen ELISA; and microbiology\/histopathology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Overall success rate of molecular genetic testing\u2010guided salvage treatments was low (68%); none of the regimens chosen was significantly better than another. Multivariable logistic regression analysis did not reveal any factors that may predict treatment failure.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Eradication success was poor despite susceptibility testing. Gastroenterologists are advised to prescribe recommended salvage treatments, considering recommended dosages and prolonged treatment duration.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12494","type":"journal-article","created":{"date-parts":[[2018,6,6]],"date-time":"2018-06-06T11:39:35Z","timestamp":1528285175000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Retrospective study on outcome of salvage <i>Helicobacter pylori<\/i> eradication therapies based on molecular genetic susceptibility testing"],"prefix":"10.1111","volume":"23","author":[{"given":"Benjamin","family":"Bl\u00fcmel","sequence":"first","affiliation":[{"name":"Faculty of Medicine Institute of Medical Microbiology and Hygiene Medical Center\u2010University of Freiburg  Freiburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hanna","family":"Goelz","sequence":"additional","affiliation":[{"name":"Faculty of Medicine 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