{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,28]],"date-time":"2026-10-28T14:30:51Z","timestamp":1793197851235,"version":"4.5.0"},"reference-count":8,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2018,8,28]],"date-time":"2018-08-28T00:00:00Z","timestamp":1535414400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"R-Biopharm","id":[{"id":"https:\/\/ror.org\/040ppgc54","id-type":"ROR","asserted-by":"crossref"}]},{"name":"Mobidiag"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,10]]},"abstract":"Abstract<\/jats:title>\n \n Background<\/jats:title>\n \n Knowledge of antimicrobial susceptibility, especially to macrolides, has become crucial for the management of\n Helicobacter pylori<\/jats:italic>\n infection. Our aim was to evaluate two new PCR kits able to detect\n H.\u00a0pylori<\/jats:italic>\n in gastric biopsies as well as the mutations associated with macrolide resistance.\n <\/jats:p>\n <\/jats:sec>\n \n Materials and Methods<\/jats:title>\n \n Two hundred successive biopsies (received from gastroenterologists all over France) were used. The two new kits tested were Amplidiag\n H.\u00a0pylori<\/jats:italic>\n +Clari\n R<\/jats:sup>\n from Mobidiag Espoo, Finland, and RIDA\n \u00ae<\/jats:sup>\n GENE\n H.\u00a0pylori<\/jats:italic>\n from R\u2010Biopharm, Darmstadt, Germany. Culture and a validated in\u2010house real\u2010time PCR were also performed, and in the case of a positive culture, Etest for clarithromycin was carried out. Discrepancies were solved by looking at the pathologic data.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n Culture was positive in 68 cases (34%), and with our in\u2010house real\u2010time PCR in these 68 cases plus 5 others (N\u00a0=\u00a073, 36%). All were also detected by the two new kits. In addition, RIDA\n \u00ae<\/jats:sup>\n GENE\n H.\u00a0pylori<\/jats:italic>\n detected one more positive also detected by Amplidiag\n H.\u00a0pylori<\/jats:italic>\n +Clari\n R<\/jats:sup>\n , and Amplidiag detected two other positives. Of these three additional cases, pathology confirmed the positivity for two. Only one case diagnosed by Amplidiag could be considered as a false positive. With regard to clarithromycin resistance, 22 cases were detected. The corresponding mutations (A2142\/43G) were all identified with the three PCRs.\n <\/jats:p>\n <\/jats:sec>\n \n Conclusions<\/jats:title>\n \n These two new kits which have an excellent sensitivity and specificity are convenient to use, adaptable to different thermocyclers, provide quick results, and deserve to be used in\n H.\u00a0pylori<\/jats:italic>\n diagnosis for a better choice of treatment regimen.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/hel.12512","type":"journal-article","created":{"date-parts":[[2018,8,30]],"date-time":"2018-08-30T02:30:48Z","timestamp":1535596248000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":63,"title":["Real\u2010time PCR for\n Helicobacter pylori<\/i>\n diagnosis. The best tools available"],"prefix":"10.1111","volume":"23","author":[{"given":"Lucie","family":"B\u00e9n\u00e9jat","sequence":"first","affiliation":[{"name":"French National Reference Centre for Campylobacters & Helicobacters H\u00f4pital Pellegrin Bordeaux France"},{"name":"INSERM UMR1053 Bordeaux BaRITOn University Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Ducournau","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Campylobacters & Helicobacters H\u00f4pital Pellegrin Bordeaux France"},{"name":"INSERM UMR1053 Bordeaux BaRITOn University Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Philippe","family":"Lehours","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Campylobacters & Helicobacters H\u00f4pital Pellegrin Bordeaux France"},{"name":"INSERM UMR1053 Bordeaux BaRITOn University Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-1612","authenticated-orcid":false,"given":"Francis","family":"M\u00e9graud","sequence":"additional","affiliation":[{"name":"French National Reference Centre for Campylobacters & Helicobacters H\u00f4pital Pellegrin Bordeaux France"},{"name":"INSERM UMR1053 Bordeaux BaRITOn University Bordeaux Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2018,8,28]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2036.2002.01169.x"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2016-312288"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.397-402.2003"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.06.003"},{"key":"e_1_2_7_6_1","doi-asserted-by":"crossref","first-page":"1007","DOI":"10.1128\/jcm.22.6.1007-1010.1985","article-title":"Characterization of \u201cCampylobacter pyloridis\u201d by culture, enzymatic profile, and protein content","volume":"22","author":"Megraud F","year":"1985","journal-title":"J Clin Microbiol"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/CMR.00033-06"},{"key":"e_1_2_7_8_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.35.4.995-998.1997"},{"key":"e_1_2_7_9_1","article-title":"Diagnostic et traitement de l'infection \u00e0 Helicobacter pylori en centre hospitalier g\u00e9n\u00e9ral. Apport de la PCR et de la culture pour le diagnostic et premiers r\u00e9sultats des traitements orient\u00e9s selon les recommandations du GEFH","author":"Heluwaert F","year":"2018","journal-title":"Journ\u00e9es Francophones d'H\u00e9pato\u2010gastroent\u00e9rologie & d'Oncologie Digestive"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhel.12512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12512","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/hel.12512","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12512","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,9,10]],"date-time":"2023-09-10T16:53:40Z","timestamp":1694364820000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hel.12512"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2018,8,28]]},"references-count":8,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2018,10]]}},"alternative-id":["10.1111\/hel.12512"],"URL":"https:\/\/doi.org\/10.1111\/hel.12512","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2018,8,28]]},"assertion":[{"value":"2018-08-28","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12512"}}