{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,26]],"date-time":"2026-09-26T00:58:19Z","timestamp":1790384299646,"version":"4.1.0"},"reference-count":82,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,9,23]],"date-time":"2018-09-23T00:00:00Z","timestamp":1537660800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100009045","name":"Uniwersytet Jagiello\u0144ski Collegium Medicum","doi-asserted-by":"publisher","award":["K\/ZDS\/005736"],"award-info":[{"award-number":["K\/ZDS\/005736"]}],"id":[{"id":"10.13039\/100009045","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2018,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Major human gastrointestinal pathogen <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H.\u00a0pylori<\/jats:italic>) colonizes the gastric mucosa causing inflammation and severe complications including cancer, but the involvement of fibroblasts in the pathogenesis of these disorders in <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010infected stomach has been little studied. Normal stroma contains few fibroblasts, especially myofibroblasts. Their number rapidly increases in the reactive stroma surrounding inflammatory region and neoplastic tissue; however, the interaction between <jats:italic>H.\u00a0pylori<\/jats:italic> and fibroblasts remains unknown. We determined the effect of coincubation of normal rat gastric fibroblasts with alive <jats:italic>H.\u00a0pylori<\/jats:italic> (cagA+vacA+) and <jats:italic>H.\u00a0pylori<\/jats:italic> (cagA\u2212vacA\u2212) strains on the differentiation of these fibroblasts into cells possessing characteristics of cancer\u2010associated fibroblasts (CAFs) able to induce epithelial\u2010mesenchymal transition (EMT) of normal rat gastric epithelial cells (RGM\u20101).<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>The panel of CAFs markers mRNA was analyzed in <jats:italic>H.\u00a0pylori (cagA+vacA+)<\/jats:italic>\u2010infected fibroblasts by RT\u2010PCR. After insert coculture of differentiated fibroblasts with RGM\u20101 cells from 24 up to 48, 72, and 96\u00a0hours, the mRNA expression for EMT\u2010associated genes was analyzed by RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The mRNA expression for CAFs markers was significantly increased after 72\u00a0hours of infection with <jats:italic>H.\u00a0pylori<\/jats:italic> (cagA+vacA+) but not <jats:italic>H.\u00a0pylori<\/jats:italic> (cagA\u2212vacA\u2212) strain. Following coculture with CAFs, RGM\u20101 cells showed significant decrease in E\u2010cadherin mRNA, and the parallel increase in the expression of Twist and Snail transcription factors mRNA was observed along with the overexpression of mRNAs for TGF\u03b2R, HGFR, FGFR, N\u2010cadherin, vimentin, \u03b1\u2010SMA, VEGF, and integrin\u2010\u03b21.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (cagA+vacA+) strain induces differentiation of normal fibroblasts into CAFs, likely to initiate the EMT process in RGM\u20101 epithelial cell line.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12538","type":"journal-article","created":{"date-parts":[[2018,9,24]],"date-time":"2018-09-24T03:14:18Z","timestamp":1537758858000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":43,"title":["Role of <i>Helicobacter pylori<\/i> infection in cancer\u2010associated 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