{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:23:55Z","timestamp":1792787035266,"version":"4.5.0"},"reference-count":32,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T00:00:00Z","timestamp":1549497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Shenzhen Key Technology R&D Program","award":["JSGG20170413152936281"],"award-info":[{"award-number":["JSGG20170413152936281"]}]},{"name":"High-level Talent Development Plan of Hebei Province"},{"name":"Hebei Province Science and Technology Research and Development Project","award":["162777113D"],"award-info":[{"award-number":["162777113D"]}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Objective<\/jats:title>\n                    <jats:p>\n                      Infection with\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      (\n                      <jats:italic>H pylori<\/jats:italic>\n                      ), especially cytotoxin\u2010associated gene A\u2010positive (CagA+) strains, has been associated with various gastrointestinal and extragastric diseases. The aim of this study was to characterize\n                      <jats:italic>H pylori<\/jats:italic>\n                      \u2010induced alterations in the gastric and tongue coating microbiota and evaluate their potential impacts on human health.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Design<\/jats:title>\n                    <jats:p>\n                      The gastric mucosa and tongue coating specimens were collected from 80 patients with chronic gastritis, and microbiota profiles were generated by 16S rRNA gene sequencing. Samples were grouped as\n                      <jats:italic>H pylori negative<\/jats:italic>\n                      (n = 32), CagA\u2010negative\n                      <jats:italic>H pylori infection<\/jats:italic>\n                      (n = 13), and CagA\u2010positive\n                      <jats:italic>H pylori infection<\/jats:italic>\n                      (n=35). The comparison of bacterial relative abundance was made using a generalized linear model. Functional profiling of microbial communities was predicted with PICRUSt and BugBase. Microbial correlation networks were produced by utilizing SparCC method.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Significant alterations of the gastric microbiota were found in the\n                      <jats:italic>H pylori<\/jats:italic>\n                      +\/CagA+ samples, represented by a decrease in bacterial diversity, a reduced abundance of\n                      <jats:italic>Roseburia<\/jats:italic>\n                      , and increased abundances of\n                      <jats:italic>Helicobacter<\/jats:italic>\n                      and\n                      <jats:italic>Haemophilus<\/jats:italic>\n                      genera. At the community level, functions involved in biofilm forming, mobile element content, and facultative anaerobiosis were significantly decreased in gastric microbiome of the\n                      <jats:italic>H pylori<\/jats:italic>\n                      + subjects. The presence of CagA gene was linked to an increased proportion of Gram\u2010negative bacteria in the stomach, thereby contributing to an upregulation of lipopolysaccharide (LPS) biosynthesis. The number of bacterial interactions was greatly reduced in networks of both tongue coating and gastric microbiota of the\n                      <jats:italic>H pylori<\/jats:italic>\n                      +\/CagA+ subject, and the cooperative bacterial interactions dominated the tongue coating microbiome.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Infection with\n                      <jats:italic>H pylori<\/jats:italic>\n                      strains possessing CagA may increase the risk of various diseases, by upregulating LPS biosynthesis in the stomach and weakening the defense of oral microbiota against microorganisms with pathogenic potential.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hel.12567","type":"journal-article","created":{"date-parts":[[2019,2,8]],"date-time":"2019-02-08T01:56:06Z","timestamp":1549590966000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":94,"title":["<i>Helicobacter pylori<\/i>\n                    infection alters gastric and tongue coating microbial communities"],"prefix":"10.1111","volume":"24","author":[{"given":"Yubin","family":"Zhao","sequence":"first","affiliation":[{"name":"The Traditional Chinese Medicine Hospital of Shijiazhuang Affiliated to Hebei University of Chinese Medicine Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1904-392X","authenticated-orcid":false,"given":"Xuefeng","family":"Gao","sequence":"additional","affiliation":[{"name":"Shenzhen University General Hospital Shenzhen China"},{"name":"Shenzhen University Clinical Medical Academy Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jiaxuan","family":"Guo","sequence":"additional","affiliation":[{"name":"The Traditional Chinese Medicine Hospital of Shijiazhuang Affiliated to Hebei University of Chinese Medicine Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dongbao","family":"Yu","sequence":"additional","affiliation":[{"name":"Shenzhen Hoiracle Bio\u2010Tech Co., Ltd. Shenzhen China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ying","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Bioengineering, School of Chemical Engineering Shijiazhuang University Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Huijie","family":"Wang","sequence":"additional","affiliation":[{"name":"The Traditional Chinese Medicine Hospital of Shijiazhuang Affiliated to Hebei University of Chinese Medicine Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuchan","family":"Li","sequence":"additional","affiliation":[{"name":"The Traditional Chinese Medicine Hospital of Shijiazhuang Affiliated to Hebei University of Chinese Medicine Shijiazhuang China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2019,2,7]]},"reference":[{"key":"e_1_2_7_2_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506655103"},{"key":"e_1_2_7_3_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0002836"},{"key":"e_1_2_7_4_1","doi-asserted-by":"publisher","DOI":"10.1038\/srep18594"},{"key":"e_1_2_7_5_1","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2017.02508"},{"key":"e_1_2_7_6_1","doi-asserted-by":"publisher","DOI":"10.1136\/gutjnl-2017-314281"},{"key":"e_1_2_7_7_1","doi-asserted-by":"publisher","DOI":"10.1038\/mi.2016.131"},{"key":"e_1_2_7_8_1","first-page":"1","article-title":"Schistosomes, liver flukes and Helicobacter pylori. 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