{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,5]],"date-time":"2026-08-05T05:36:27Z","timestamp":1785908187177,"version":"3.56.0"},"reference-count":53,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,4,5]],"date-time":"2019-04-05T00:00:00Z","timestamp":1554422400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001700","name":"Ministry of Education, Culture, Sports, Science and Technology","doi-asserted-by":"publisher","award":["221S0002, 16H06279, 16H05191 and 18KK0266"],"award-info":[{"award-number":["221S0002, 16H06279, 16H05191 and 18KK0266"]}],"id":[{"id":"10.13039\/501100001700","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["DK62813, P30 DK56338, U19 AI116497"],"award-info":[{"award-number":["DK62813, P30 DK56338, U19 AI116497"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2019,6]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Lack of a model that mirrors <jats:italic>Helicobacter pylori\u2010<\/jats:italic>induced gastric mucosal inflammation has hampered investigation of early host\u2010bacterial interactions. We used an ex vivo model of human stomach, gastric epithelial organoid monolayers (gastroid monolayers) to investigate interactions of <jats:italic>H\u00a0pylori<\/jats:italic> infection and the apical junctional complex and interleukin\u20108 (IL\u20108) expression.<\/jats:p><\/jats:sec><jats:sec><jats:title>Method<\/jats:title><jats:p>Morphology of human antral mucosal gastroid monolayers was evaluated using histology, immunohistochemical (IHC) staining, and transmission electron microscopy (TEM). Functional and gross changes in the apical junctional complexes were assessed using transepithelial electrical resistance (TEER), cytotoxicity assays, and confocal laser scanning microscopy. IL\u20108 expression was evaluated by real\u2010time quantitative PCR and ELISA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>When evaluated by IHC and TEM, the morphology of gastroid monolayers closely resembled in vivo human stomach. Following inoculation of <jats:italic>H\u00a0pylori<\/jats:italic>, TEER transiently declined (up to 51%) in an <jats:italic>H\u00a0pylori<\/jats:italic> density\u2010dependent manner. TEER recovered by 48\u00a0hours post\u2010infection and remained normal despite continued presence and replication of <jats:italic>H\u00a0pylori<\/jats:italic>. Confocal scanning microscopy showed minimal disruption of zonula occludens\u20101 or E\u2010cadherin structure. IL\u20108 production was unchanged by infection with either CagA\u2010positive or CagA\u2010negative <jats:italic>H\u00a0pylori <\/jats:italic>and JNK and MEK inhibitors did not suppress IL\u20108 production, whereas p38 and IKK inhibitor significantly did.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Human gastroid monolayers provide a model for experimental <jats:italic>H\u00a0pylori<\/jats:italic> infection more consistent with in vivo human infections than seen with typical gastric epithelial cell lines. This ex vivo system should lead to better understanding of <jats:italic>H\u00a0pylori<\/jats:italic> host\u2010pathogen interactions.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12583","type":"journal-article","created":{"date-parts":[[2019,4,6]],"date-time":"2019-04-06T01:36:12Z","timestamp":1554514572000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":20,"title":["Changes of tight junction and interleukin\u20108 expression using a human gastroid monolayer model of <i>Helicobacter pylori<\/i> infection"],"prefix":"10.1111","volume":"24","author":[{"given":"Takahiro","family":"Uotani","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Baylor College of Medicine and Michael E. DeBakey Veterans Affairs Medical Center Houston Texas"},{"name":"Department of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kosuke","family":"Murakami","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tomohisa","family":"Uchida","sequence":"additional","affiliation":[{"name":"Department of Molecular Pathology Oita University Faculty of Medicine Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shingo","family":"Tanaka","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Baylor College of Medicine and Michael E. DeBakey Veterans Affairs Medical Center Houston Texas"},{"name":"Department of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroyuki","family":"Nagashima","sequence":"additional","affiliation":[{"name":"Gastroenterological Medicine Rumoi City Hospital Rumoi Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xi\u2010Lei","family":"Zeng","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Junko","family":"Akada","sequence":"additional","affiliation":[{"name":"Department of Environmental and Preventive Medicine Oita University Faculty of Medicine Yufu Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mary K.","family":"Estes","sequence":"additional","affiliation":[{"name":"Department of Molecular Virology and Microbiology Baylor College of Medicine Houston Texas"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6908-8317","authenticated-orcid":false,"given":"David Y.","family":"Graham","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Baylor College of Medicine and Michael E. 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