{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,28]],"date-time":"2026-09-28T22:08:03Z","timestamp":1790633283835,"version":"4.1.0"},"reference-count":48,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T00:00:00Z","timestamp":1577318400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2020,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      In this study, one\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      isolate, from gastric biopsy of a dyspeptic patient that turned into mucoid\u2010coccoid (MC) form upon consecutive subcultures, was identified. The culturability, antibiotic resistance, and lipid contents of MC were compared with those of non\u2010mucoid (NM) spiral\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      .\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      Mucoid\u2010coccoid and NM\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      were subcultured on Brucella blood agar (BBA) and incubated under aerobic and microaerobic atmospheres at 37\u00b0C. Cultures were examined for colony characteristics and bacterial morphology after 1\u20103\u00a0days. The isolates were identified by biochemical tests and detection of\n                      <jats:italic>H\u00a0pylori\u2010<\/jats:italic>\n                      16S rDNA. Antibiogram was performed with currently used antibiotics for\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      eradication. Cellular lipid contents were extracted and analyzed by gas chromatography.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Compared with pin\u2010pointed and glistening colonies of NM\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      that appeared under microaerobic conditions, MC\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      grew well in consecutive subcultures under aerobic and microaerobic atmospheres and produced white patches of mucoid colonies. MC exhibited coccoid and NM spiral morphology. Both isolates were catalase, oxidase, and urease positive and contained 16S rDNA. Compared with NM that was susceptible to almost all the antibiotics, MC was resistant to all the antibiotics. Lipid analyses showed high frequency of unsaturated fatty acids and cholesterol in MC.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>\n                      Coccoid forms with high fatty acid and cholesterol contents that show resistance to antibiotics might resist against other stressful conditions such as gastric acidity and immune response. Moreover, mucoid property may enhance resistance of coccoids to stresses. With mucoid\u2010coccoid lifestyle,\n                      <jats:italic>H\u00a0pylori<\/jats:italic>\n                      may establish a chronic infection refractory to antimicrobial therapy.\n                    <\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hel.12678","type":"journal-article","created":{"date-parts":[[2019,12,26]],"date-time":"2019-12-26T21:41:08Z","timestamp":1577396468000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":55,"title":["Mucoid and coccoid\n                    <i>Helicobacter pylori<\/i>\n                    with fast growth and antibiotic resistance"],"prefix":"10.1111","volume":"25","author":[{"given":"Sara","family":"Kadkhodaei","sequence":"first","affiliation":[{"name":"Department of Microbiology School of Biology University College of Sciences Tehran University Tehran 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