{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,12]],"date-time":"2026-05-12T15:38:49Z","timestamp":1778600329309,"version":"3.51.4"},"reference-count":29,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T00:00:00Z","timestamp":1602892800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004581","name":"Brandenburger Staatsministerium f\u00fcr Wissenschaft, Forschung und Kultur","doi-asserted-by":"publisher","award":["\"Konsequenzen der altersassoziierten Zell\u2010 und Org"],"award-info":[{"award-number":["\"Konsequenzen der altersassoziierten Zell\u2010 und Org"]}],"id":[{"id":"10.13039\/501100004581","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Antimicrobial therapy is recommended to eradicate <jats:italic>Helicobacter (H.) pylori<\/jats:italic> in infected individuals. As first\u2010line treatments are empiric, knowledge of antimicrobial resistance is key to successful eradication.<\/jats:p><\/jats:sec><jats:sec><jats:title>Aims<\/jats:title><jats:p>We investigated primary resistance in an eastern German region to derive recommendations for eradication treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We used molecular genetic methods to examine Helicobacter rapid urease test (RUT) positive gastric specimens of 533 patients from Berlin and the federal state of Brandenburg with allegedly no prior eradication treatment. Tissue samples were removed from RUT and screened by real\u2010time PCR for mutations conferring resistance to clarithromycin. In addition, 182 samples out of 533 were tested for resistance to levofloxacin and tetracycline.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Primary resistances were 10.9% (58 out of 533) to clarithromycin; 13.7% (25\/182) to levofloxacin; and 2.2% to tetracycline (4\/182). Combined resistance to clarithromycin\/levofloxacin was low (2.2%, 4\/182). Female sex was significantly associated with clarithromycin resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Clarithromycin may be a suitable first\u2010line antibiotic for about 90% of outpatients. A simple molecular test may help physicians avoid prescription of an ineffective first\u2010line regimen.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12765","type":"journal-article","created":{"date-parts":[[2020,10,17]],"date-time":"2020-10-17T07:44:16Z","timestamp":1602920656000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Antimicrobial resistance of <i>Helicobacter pylori<\/i> in an eastern German region"],"prefix":"10.1111","volume":"26","author":[{"given":"Dirk","family":"Hofreuter","sequence":"first","affiliation":[{"name":"Department of Biological Safety German Federal Institute for Risk Assessment  Berlin Germany"},{"name":"Brandenburg Medical School Institute of Laboratory Medicine Brandenburg Hospital  Brandenburg Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Johannes","family":"Behrendt","sequence":"additional","affiliation":[{"name":"Practice for Gastroenterology  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