{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,26]],"date-time":"2026-06-26T06:52:24Z","timestamp":1782456744142,"version":"3.54.5"},"reference-count":54,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2020,10,18]],"date-time":"2020-10-18T00:00:00Z","timestamp":1602979200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2021,2]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic>, a Gram\u2010negative bacterium, is associated with a wide range of gastric diseases such as gastritis, duodenal ulcer, and gastric cancer. The prevalence of <jats:italic>H\u00a0pylori<\/jats:italic> and risk of disease vary in different parts of the world based on the prevailing bacterial lineage. Here, we present a contextual and comparative genomics analysis of 20 clinical isolates of <jats:italic>H pylori<\/jats:italic> from patients in Bangladesh. Despite a uniform host ethnicity (Bengali), isolates were classified as being part of the HpAsia2 (50%) or HpEurope (50%) population. Out of twenty isolates, eighteen isolates were <jats:italic>cagA<\/jats:italic> positive, with two HpEurope isolates being <jats:italic>cagA<\/jats:italic> negative, three EPIYA motif patterns (AB, AB\u2010C, and ABC\u2010C) were observed among the <jats:italic>cagA<\/jats:italic>\u2010positive isolates. Three <jats:italic>vacA<\/jats:italic> genotypes were observed with the s1m1i1dic1 genotype observed in 75% of isolates; the s1m2i1d1c2 and s2m2i2d2c2 genotypes were found to be 15% and 10% of isolates, respectively. The non\u2010virulent genotypes s2m2i2d2c2 was only observed in HpEurope population isolates. Genotypic analysis of <jats:italic>oipA<\/jats:italic> gene, present in all isolates, revealed five different patterns of the CT repeat; all HpAsia2 isolates were in \u201cON\u201d while 20% of HpEurope isolates were genotypically \u201cOFF.\u201d The three blood group antigen binding adhesins encoded genes (<jats:italic>bab<\/jats:italic> genes) examined and we observed that the most common genotype was (<jats:italic>babA<\/jats:italic>\/<jats:italic>babB<\/jats:italic>\/\u2010) found in eight isolates, notably six were HpAsia2 isolates. The <jats:italic>babA<\/jats:italic> gene was found in all HpAsia2 isolates but present in only half of the HpEurope isolates. In silico antibiotic susceptibility analysis revealed that 40% of the strains were multi\u2010drug resistant. Mutations associated with resistance to metronidazole, fluoroquinolone, and clarithromycin were detected 90%, 45%, and 5%, respectively, in <jats:italic>H pylori<\/jats:italic> strain. In conclusion, it is evident that two populations of <jats:italic>H\u00a0pylori<\/jats:italic> with similar antibiotic profiles are predominant in Bangladesh, and it appears that genotypically the HpAisa2 isolates are potentially more virulent than the HpEurope isolates.<\/jats:p>","DOI":"10.1111\/hel.12766","type":"journal-article","created":{"date-parts":[[2020,10,19]],"date-time":"2020-10-19T01:33:55Z","timestamp":1603071235000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["A comparative whole genome analysis of <i>Helicobacter pylori<\/i> from a human dense South Asian setting"],"prefix":"10.1111","volume":"26","author":[{"given":"Shamsul","family":"Qumar","sequence":"first","affiliation":[{"name":"Department of Biotechnology and Bioinformatics University of Hyderabad  Hyderabad India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Trang Hoa","family":"Nguyen","sequence":"additional","affiliation":[{"name":"The Hospital for Tropical Diseases Oxford University Clinical Research Unit  Ho Chi Minh City Vietnam"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shamsun","family":"Nahar","sequence":"additional","affiliation":[{"name":"International Center for Diarrhoeal Disease Research Bangladesh  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nishat","family":"Sarker","sequence":"additional","affiliation":[{"name":"International Center for Diarrhoeal Disease Research Bangladesh  Dhaka Bangladesh"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stephen","family":"Baker","sequence":"additional","affiliation":[{"name":"Cambridge Institute of Therapeutic Immunology &amp; Infectious Disease Cambridge University  Cambridge 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