{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,14]],"date-time":"2026-10-14T00:56:07Z","timestamp":1791939367478,"version":"4.3.4"},"reference-count":9,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T00:00:00Z","timestamp":1633824000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2021,12]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background and Objectives<\/jats:title><jats:p>The present study describes the successful adaptation of an in\u2010house Polymerase Chain Reaction (PCR) for <jats:italic>Helicobacter pylori<\/jats:italic> detection coupled with the main mutations associated with resistance to clarithromycin in ready\u2010to\u2010use PCR microwell strips.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>These microwell strips can be used on LightCycler\u00ae 480, and are delivered with nine microliters of the reaction mixture dispensed into 8\u2010well microwell strips. An extraction control PCR targeting the \u03b2\u2010globin household gene is amplified in the same run as <jats:italic>H\u00a0pylori<\/jats:italic> detection.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results and Conclusion<\/jats:title><jats:p>These microwell strips can be stored at \u221220\u00b0C for 1\u00a0year and left at room temperature and in the light for up to 4\u00a0h with no impact on the PCR results. Microwell strips can also undergo a thaw and refreeze cycle without impacting the PCR results. These PCR microwell strips are available for purchase from Eurogentec.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12855","type":"journal-article","created":{"date-parts":[[2021,10,10]],"date-time":"2021-10-10T15:29:32Z","timestamp":1633879772000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":13,"title":["Adaptation of an in\u2010house PCR for the detection of <i>Helicobacter pylori<\/i> and the mutations associated with macrolide resistance into ready\u2010to\u2010use PCR microwell strips"],"prefix":"10.1111","volume":"26","author":[{"given":"Lucie","family":"B\u00e9n\u00e9jat","sequence":"first","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Astrid","family":"Ducournau","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chlo\u00e9","family":"Domingues\u2010Martins","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00e9lanie","family":"Lecoeur","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice","family":"Blosse","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"},{"name":"Univ. Bordeaux INSERM UMR1053 Bordeaux Research in Translational Oncology BaRITOn Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2481-1612","authenticated-orcid":false,"given":"Francis","family":"M\u00e9graud","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"},{"name":"Univ. Bordeaux INSERM UMR1053 Bordeaux Research in Translational Oncology BaRITOn Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emilie","family":"Bess\u00e8de","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"},{"name":"Univ. Bordeaux INSERM UMR1053 Bordeaux Research in Translational Oncology BaRITOn Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6077-1825","authenticated-orcid":false,"given":"Philippe","family":"Lehours","sequence":"additional","affiliation":[{"name":"French National Reference Center for Campylobacters &amp; Helicobacters Bordeaux Hospital University Center Bordeaux France"},{"name":"Univ. Bordeaux INSERM UMR1053 Bordeaux Research in Translational Oncology BaRITOn Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2021,10,10]]},"reference":[{"key":"e_1_2_9_2_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1523-5378.2007.00486.x"},{"key":"e_1_2_9_3_1","doi-asserted-by":"publisher","DOI":"10.3390\/jcm8010053"},{"key":"e_1_2_9_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12512"},{"key":"e_1_2_9_5_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.02352-07"},{"key":"e_1_2_9_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1574-6968.1998.tb13172.x"},{"key":"e_1_2_9_7_1","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.41.1.397-402.2003"},{"key":"e_1_2_9_8_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cmi.2016.06.003"},{"key":"e_1_2_9_9_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12702"},{"key":"e_1_2_9_10_1","doi-asserted-by":"publisher","DOI":"10.1111\/hel.12560"}],"container-title":["Helicobacter"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12855","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/full-xml\/10.1111\/hel.12855","content-type":"application\/xml","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hel.12855","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,8,28]],"date-time":"2023-08-28T16:59:30Z","timestamp":1693241970000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hel.12855"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,10,10]]},"references-count":9,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2021,12]]}},"alternative-id":["10.1111\/hel.12855"],"URL":"https:\/\/doi.org\/10.1111\/hel.12855","archive":["Portico"],"relation":{},"ISSN":["1083-4389","1523-5378"],"issn-type":[{"value":"1083-4389","type":"print"},{"value":"1523-5378","type":"electronic"}],"subject":[],"published":{"date-parts":[[2021,10,10]]},"assertion":[{"value":"2021-09-07","order":0,"name":"received","label":"Received","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-09-23","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2021-10-10","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}],"article-number":"e12855"}}