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However, the role and mechanism of macrophage dysfunction in <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010associated pediatric gastritis remain unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>An RNA\u2010sequencing assay was used to examine the differential gene expression in normal gastric antrum, non\u2010<jats:italic>H.\u00a0pylori\u2010<\/jats:italic>infected tissue, and <jats:italic>H.\u00a0pylori\u2010<\/jats:italic>infected pediatric gastritis tissue. qPCR assays were applied to verify the expression of target genes. HE staining was performed to identify the occurrence of inflammation in the normal gastric antrum, non\u2010<jats:italic>H.\u00a0pylori\u2010<\/jats:italic>infected tissue, <jats:italic>and H.\u00a0pylori\u2010<\/jats:italic>infected pediatric gastritis tissue. Western blotting was used to measure the expression of SHP2 in pediatric gastritis tissue. The metabolic profile of macrophages was determined via Seahorse metabolic analysis. Flow cytometry analysis was used to examine the level of reactive oxygen species (ROS).<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>H.\u00a0pylori<\/jats:italic> \u2010infected gastritis tissue exhibited many differentially expressed genes (DEGs) compared to gastritis tissue without <jats:italic>H.\u00a0pylori<\/jats:italic> infection. Moreover, <jats:italic>H.\u00a0pylori<\/jats:italic> \u2010infected gastritis tissue showed many DEGs annotated with an overactive immune response. We identified that tyrosine\u2010protein phosphatase nonreceptor type 11 (<jats:italic>PTPN11<\/jats:italic>), which encodes SHP2, was significantly increased in macrophages of <jats:italic>H.\u00a0pylori<\/jats:italic> \u2010infected gastritis tissue. Furthermore, we revealed that SHP2 could activate the glycolytic function of macrophages to promote <jats:italic>H.\u00a0pylori<\/jats:italic> \u2010induced inflammation. The transcription factor <jats:italic>SPI1<\/jats:italic> , as the downstream molecule of SHP2, could be responsible for the regulation of metabolism\u2010associated gene expression and inflammation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Our study illustrated the molecular landscape of <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010infected gastritis tissue in children and suggested that the SHP2\/<jats:italic>SPI1<\/jats:italic>axis could be a novel therapeutic target in <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010induced pediatric gastritis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12895","type":"journal-article","created":{"date-parts":[[2022,4,19]],"date-time":"2022-04-19T08:45:14Z","timestamp":1650357914000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["<scp>SHP2<\/scp>\/<i>SPI1<\/i>axis promotes glycolysis and the inflammatory response of macrophages in <i>Helicobacter pylori<\/i>\u2010induced pediatric gastritis"],"prefix":"10.1111","volume":"27","author":[{"given":"Chuanying","family":"Li","sequence":"first","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Sciences Anhui Medical University  Hefei China"},{"name":"Department of Gastroenterology Children's Hospital of Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Changyun","family":"Lu","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Sciences Anhui Medical University  Hefei China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Liangju","family":"Gong","sequence":"additional","affiliation":[{"name":"Department of Pathophysiology, School of Basic Medical Sciences Anhui Medical University  Hefei 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