{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:03:06Z","timestamp":1778695386925,"version":"3.51.4"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T00:00:00Z","timestamp":1661731200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.82000531and82170580"],"award-info":[{"award-number":["No.82000531and82170580"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["No.82000531and82170580"],"award-info":[{"award-number":["No.82000531and82170580"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2022,10]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>The oral cavity is considered a potential reservoir of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H.\u00a0pylori<\/jats:italic>), and the imbalance of oral microbiota directly reflects the health of the host. We aimed to explore the relationship among oral microbiota, <jats:italic>H.\u00a0pylori<\/jats:italic> infection, and vonoprazan\u2010amoxicillin (VA) dual therapy for <jats:italic>H.\u00a0pylori<\/jats:italic> eradication.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic>\u2010positive patients were randomized into low\u2010 or high\u2010dose VA dual therapy (i.e., amoxicillin 1\u00a0g b.i.d. or t.i.d. and vonoprazan 20\u2009mg b.i.d) for 7 or 10\u2009days. <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010negative patients served as normal controls. Saliva samples were collected from 41 <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive patients and 13 <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010negative patients. The oral microbiota was analyzed by 16S rRNA gene sequencing, followed by bioinformatics analysis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic>\u2010positive patients had higher richness and diversity and better evenness of oral microbiota than normal controls. Beta diversity analysis estimated by Bray\u2013Curtis or weighted UniFrac showed distinct clustering between <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive patients and normal controls. The number of bacterial interactions was reduced in <jats:italic>H.\u00a0pylori<\/jats:italic>\u2010positive patients compared with that in negative patients. Forty\u2010one patients evaluated before and after successful <jats:italic>H.\u00a0pylori<\/jats:italic> eradication were divided into low (L\u2010VA) and high dose (H\u2010VA) amoxicillin dose groups. The alpha and beta diversity of the oral microbiota between L\u2010VA and H\u2010VA patients exhibited no differences at the three time points (before eradication, after eradication, and at confirmation of <jats:italic>H.\u00a0pylori<\/jats:italic> infection cure).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> infection could alter the diversity, composition, and bacterial interactions of the oral microbiota. Both L\u2010VA and H\u2010VA dual therapy showed minimal influence on the oral microbiota.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12923","type":"journal-article","created":{"date-parts":[[2022,8,29]],"date-time":"2022-08-29T16:35:04Z","timestamp":1661790904000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Analysis of oral microbiota alterations induced by <i>Helicobacter pylori<\/i> infection and vonoprazan\u2010amoxicillin dual therapy for <i>Helicobacter pylori<\/i> eradication"],"prefix":"10.1111","volume":"27","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2708-7612","authenticated-orcid":false,"given":"Yi","family":"Hu","sequence":"first","affiliation":[{"name":"Department Of Gastroenterology, Digestive Disease Hospital The First Affiliated Hospital of Nanchang University  Nanchang China"},{"name":"JiangXi Clinical Research Center for Gastroenterology  Nanchang 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