{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T23:39:50Z","timestamp":1790293190933,"version":"4.1.0"},"reference-count":57,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T00:00:00Z","timestamp":1670198400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>\n                      <jats:italic>Helicobacter pylori<\/jats:italic>\n                      is an infection of concern for its chronic colonization leading to peptic ulcers and gastric cancer. In recent times, microRNAs have been extensively studied to understand their role in the pathogenesis of this bacteria in diverse contexts of gastric diseases. The current analysis reports the microRNA\u2010mRNA interactions that are associated with effective survival and virulence of this pathogen.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Materials and Methods<\/jats:title>\n                    <jats:p>\n                      We convened differentially regulated human microRNAs responsive to\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection (HP\u2010hDEmiRs) at different multiplicity of infection and time points in human gastric cell lines through retrospective data mining of experimental studies. In view of the molecular disparity of clinical samples and animal models, data from tissue, serum\/plasma, urine, and ascites were excluded. Further, we utilized diverse bioinformatics approaches to retrieve experimentally validated, high\u2010confidence targets of the HP\u2010hDEmiRs to analyze the microRNA\u2010mRNA interactions that are relevant to\n                      <jats:italic>H. pylori<\/jats:italic>\n                      pathogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      A total of 39 HP\u2010hDEmiRs that showed unidirectional expression of either overexpression or downregulation were identified to modulate 23 targets explicitly studied under this infection. We also identified 476 experimentally validated targets regulated by at least 4 of the HP\u2010hDEmiRs. In addition to the pathways prior\u2010associated with\n                      <jats:italic>H. pylori<\/jats:italic>\n                      infection, the microRNA\u2010mRNA interactome analysis identified several cellular processes and pathways highly associated with cell cycle, cell division, migration, and carcinogenesis.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>This study generated a platform to study the mechanisms utilized by this pathogen using microRNAs as surrogate.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hel.12941","type":"journal-article","created":{"date-parts":[[2022,12,5]],"date-time":"2022-12-05T09:01:25Z","timestamp":1670230885000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":15,"title":["<i>Helicobacter pylori<\/i>\n                    regulated\n                    <scp>microRNA<\/scp>\n                    map of human gastric cells"],"prefix":"10.1111","volume":"28","author":[{"given":"Poornima","family":"Ramesh","sequence":"first","affiliation":[{"name":"Centre for Systems Biology and Molecular Medicine, Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sreeranjini","family":"Babu","sequence":"additional","affiliation":[{"name":"Centre for Systems Biology and Molecular Medicine, Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shruthi","family":"Ammankallu","sequence":"additional","affiliation":[{"name":"Centre for Systems Biology and Molecular Medicine, Yenepoya Research Centre Yenepoya (Deemed to be University)  Mangalore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jalaluddin Akbar 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