{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,3]],"date-time":"2026-10-03T21:02:08Z","timestamp":1791061328856,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T00:00:00Z","timestamp":1681689600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2023,8]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>As the reduced eradication rate of <jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H. pylori<\/jats:italic>), we introduced string\u2010test and quantitative PCR (qPCR) for susceptibility\u2010guided therapy innovatively. The practicality of the string test was evaluated.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>It was an open\u2010label, non\u2010randomized, parallel, single\u2010center study, in which\u00a0subjects tested by <jats:sup>13<\/jats:sup>C\u2010 urea breath test (UBT) and string\u2010qPCR were enrolled. Based on the results of string\u2010qPCR, we calculated clarithromycin and levofloxacin resistance rates and gave <jats:sup>13<\/jats:sup>C\u2010UBT positive patients 14\u2009days susceptibility\u2010guided bismuth quadruple therapy. In the empirical therapy group, we retrospectively analyzed the treatment results of <jats:sup>13<\/jats:sup>C\u2010UBT positive patients also treated with bismuth quadruple at Shenzhen Luohu People's Hospital from January 2021 to May 2022. The eradication rate was compared between susceptibility\u2010guided therapy and empirical therapy groups.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The diagnosis of <jats:italic>H. pylori<\/jats:italic> infection using the string\u2010qPCR had an overall concordance rate of 95.9% with the <jats:sup>13<\/jats:sup>C\u2010UBT results. Based on the results of string\u2010qPCR, the clarithromycin and levofloxacin resistance rates were 26.1% and 31.8%, respectively. The patients who were given 14\u2009days susceptibility\u2010guided bismuth\u2010based quadruple therapy achieved a high <jats:italic>H. pylori<\/jats:italic> eradication rate of 91.8%. Retrospective analysis of patient treatment data from January 2021 to May 2022 available in the hospital database revealed an overall success rate of 82.3% for those who received empirical bismuth\u2010based quadruple therapies, which is marginally significantly lower than that of the string\u2010qPCR susceptibility\u2010guided group (<jats:italic>p<\/jats:italic>\u2009=\u20090.084).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The high treatment success rate of 91.8% indicates that the string\u2010qPCR test is a valuable and feasible approach for clinical practice to help improve <jats:italic>H. pylori<\/jats:italic> treatment success rate.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.12985","type":"journal-article","created":{"date-parts":[[2023,4,17]],"date-time":"2023-04-17T07:54:26Z","timestamp":1681718066000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Quantitative <scp>PCR<\/scp> of string\u2010test collected gastric material: A feasible approach to detect <i>Helicobacter pylori<\/i> and its resistance against clarithromycin and levofloxacin for susceptibility\u2010guided therapy"],"prefix":"10.1111","volume":"28","author":[{"given":"Xinyuan","family":"Han","sequence":"first","affiliation":[{"name":"Department of Clinical Laboratory Anhui University of Science and Technology  Huainan Anhui China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiqiu","family":"Yu","sequence":"additional","affiliation":[{"name":"Shenzhen Luohu People's Hospital The Third Affiliated Hospital of Shenzhen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaojuan","family":"Gao","sequence":"additional","affiliation":[{"name":"Shenzhen Luohu People's Hospital The Third Affiliated Hospital of Shenzhen University  Shenzhen Guangdong 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Third Affiliated Hospital of Shenzhen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Lai","sequence":"additional","affiliation":[{"name":"Shenzhen Luohu People's Hospital The Third Affiliated Hospital of Shenzhen University  Shenzhen Guangdong China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Barry J.","family":"Marshall","sequence":"additional","affiliation":[{"name":"Marshall Laboratory of Biomedical Engineering, International Cancer Center, Laboratory of Evolutionary Theranostics (LET), School of Biomedical Engineering Shenzhen University Health Science Center  Shenzhen Guangdong China"},{"name":"Helicobacter Research Laboratory The Marshall Centre for Infectious Disease Research and Training University of Western Australia  Perth Western Australia 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