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(AMAB) Schiff base and its Cu(II) complex were identified and confirmed by different physicochemical techniques. The Schiff base (AMAB) was coordinated to copper ion through carbonyl oxygen and imine nitrogen donor sites. X\u2010ray powder diffraction shows a cubic crystal system of the Cu(II) complex. The density functional theory was used to optimize the structure geometries of the investigated compounds. The molecular docking of the active amino acids of the investigated proteins' interactions with the tested compounds was evaluated. The bactericidal or bacteriostatic effect of the compounds was screened against some bacterial strains. The activity of Cu\u2010chelate against Gram\u2010negative bacteria was mainly more effective than its (AMAB) ligand and vice versa in the case of Gram\u2010positive bacteria. The biological activity of the prepared compounds with biomolecules calf thymus DNA (CT\u2010DNA) was determined by electronic absorption spectra and DNA gel electrophoresis technique. All studies revealed that the Cu\u2010chelate derivative exhibited better binding affinity to both CT\u2010DNA than the AMAB and amoxicillin itself. The anti\u2010inflammatory effect of the designed compounds was determined by testing their protein denaturation inhibitory activity spectrophotometrically. All obtained data supported that the designed nano\u2010Cu(II) complex with Schiff base (AMAB) is a potent bactericide against <jats:italic>H. pylori<\/jats:italic>, and exhibits anti\u2010inflammatory activity. The dual inhibition effects of the designed compound represent a modern therapeutic approach with extended spectrum of action. Therefore, it can act as good drug target in antimicrobial and anti\u2010inflammtory therapies. Finally, <jats:italic>H. pylori<\/jats:italic> resistance to amoxicillin is absent or rare in many countries, thus amoxicillin nanoparticles may be beneficial for countries where amoxicillin resistance is reported.<\/jats:p>","DOI":"10.1111\/hel.13004","type":"journal-article","created":{"date-parts":[[2023,7,1]],"date-time":"2023-07-01T06:09:46Z","timestamp":1688191786000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Antimicrobial screening involving <i>Helicobacter pylori<\/i> of nano\u2010therapeutic compounds based on the amoxicillin antibiotic drug"],"prefix":"10.1111","volume":"28","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1177-2375","authenticated-orcid":false,"given":"Safaa S.","family":"Hassan","sequence":"first","affiliation":[{"name":"Department of Chemistry, Inorganic Chemistry Division, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Madonna","family":"Nader","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria","family":"Nagy","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mennatallah","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mennatulla","family":"Nader","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mina","family":"Zakaria","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nada","family":"Mohamed","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rawan","family":"Waleed","sequence":"additional","affiliation":[{"name":"Department of Biotechnology, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1646-0856","authenticated-orcid":false,"given":"Fatma B.","family":"Rashidi","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Biochemistry Division, Faculty of Science Cairo University  Giza Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2023,6,30]]},"reference":[{"key":"e_1_2_6_2_1","first-page":"77","article-title":"Antimicrobial natural products","volume":"55","author":"Rahman M","year":"2020","journal-title":"Annu Rep Med Chem"},{"key":"e_1_2_6_3_1","first-page":"369","article-title":"Antibiotic resistance","volume":"10","author":"Frieri M","year":"2017","journal-title":"J InfectPublic Health"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2016.02.009"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1080\/21505594.2016.1159366"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.2165\/00003495-197918030-00001"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1002\/j.1875-9114.1984.tb03333.x"},{"key":"e_1_2_6_8_1","first-page":"1","article-title":"StatPearls. 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