{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T04:53:53Z","timestamp":1790916833773,"version":"4.1.0"},"reference-count":25,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,3,8]],"date-time":"2024-03-08T00:00:00Z","timestamp":1709856000000},"content-version":"vor","delay-in-days":7,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/100010661","name":"Horizon 2020 Framework Programme","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100010661","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> (<jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>) infection is the most extensively studied risk factor for gastric cancer. As with any bacteria, <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic> will release distinctive odors that result from an emission of volatile metabolic byproducts in unique combinations and proportions. Effectively capturing and identifying these volatiles can pave the way for the development of innovative and non\u2010invasive diagnostic methods for determining infection. Here we characterize the <jats:italic>H. pylori<\/jats:italic> volatilomic signature, pinpoint potential biomarkers of its presence, and evaluate the variability of volatilomic signatures between different <jats:italic>H. pylori<\/jats:italic> isolates.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>Using needle trap extraction, volatiles in the headspace above <jats:italic>H. pylori<\/jats:italic> cultures were collected and, following thermal desorption at 290\u00b0C in a splitless mode, were analyzed using gas chromatography\u2013mass spectrometry. The resulting volatilomic signatures of <jats:italic>H. pylori<\/jats:italic> cultures were compared to those obtained from an analysis of the volatiles in the headspace above the cultivating medium only.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Amongst the volatiles detected, 21 showed consistent differences between the bacteria cultures and the cultivation medium, with 11 compounds being elevated and 10 showing decreased levels in the culture's headspace. The 11 elevated volatiles are four ketones (2\u2010pentanone, 5\u2010methyl\u20103\u2010heptanone, 2\u2010heptanone, and 2\u2010nonanone), three alcohols (2\u2010methyl\u20101\u2010propanol, 3\u2010methyl\u20101\u2010butanol, and 1 butanol), one aromatic (styrene), one aldehyde (2\u2010ethyl\u2010hexanal), one hydrocarbon (n\u2010octane), and one sulfur compound (dimethyl disulfide). The 10 volatiles with lower levels in the headspace of the cultures are four aldehydes (2\u2010methylpropanal, benzaldehyde, 3\u2010methylbutanal, and butanal), two heterocyclic compounds (2\u2010ethylfuran and 2\u2010pentylfuran), one ketone (2\u2010butanone), one aromatic (benzene), one alcohol (2\u2010butanol) and bromodichloromethane. Of the volatile species showing increased levels, the highest emissions are found to be for 3\u2010methyl\u20101\u2010butanol, 1\u2010butanol and dimethyl disulfide. Qualitative variations in their emissions from the different isolates was observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The volatiles emitted by <jats:italic>H. pylori<\/jats:italic> provide a characteristic volatilome signature that has the potential of being developed as a tool for monitoring infections caused by this pathogen. Furthermore, using the volatilome signature, we are able to differentiate different isolates of <jats:italic>H<\/jats:italic>. <jats:italic>pylori<\/jats:italic>. However, the volatiles also represent potential confounders for the recognition of gastric cancer volatile markers.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.13064","type":"journal-article","created":{"date-parts":[[2024,3,9]],"date-time":"2024-03-09T06:14:20Z","timestamp":1709964860000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Volatilomic signatures of different strains of <i>Helicobacter pylori<\/i>"],"prefix":"10.1111","volume":"29","author":[{"given":"Reinis","family":"Vangravs","sequence":"first","affiliation":[{"name":"Institute of Clinical and Preventive Medicine, University of Latvia  Riga Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7765-852X","authenticated-orcid":false,"given":"Linda","family":"Me\u017emale","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Preventive Medicine, University of Latvia  Riga Latvia"},{"name":"Riga East University Hospital  Riga Latvia"},{"name":"Health Centre 4  Riga Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Daria","family":"\u015alefarska\u2010Wolak","sequence":"additional","affiliation":[{"name":"Institute for Breath Research, University of Innsbruck  Innsbruck Austria"},{"name":"Institute of Chemistry, Jan Kochanowski University  Kielce Poland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Edgars","family":"Dauss","sequence":"additional","affiliation":[{"name":"Institute of Clinical and Preventive Medicine, University of Latvia  Riga Latvia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Clemens","family":"Ager","sequence":"additional","affiliation":[{"name":"Institute for Breath Research, University of Innsbruck  Innsbruck Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alejandro H.","family":"Corvalan","sequence":"additional","affiliation":[{"name":"Advanced 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