{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,4]],"date-time":"2026-03-04T08:25:17Z","timestamp":1772612717174,"version":"3.50.1"},"reference-count":41,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T00:00:00Z","timestamp":1714435200000},"content-version":"vor","delay-in-days":60,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc-nd\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2024,3]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p><jats:italic>Helicobacter pylori<\/jats:italic> infection is one of the main causes of gastric cancer. thioredoxin\u20101 (Trx1) and arginase (RocF) expressed by <jats:italic>H. pylori<\/jats:italic> were found to be closely related to its pathogenicity. However, whether Trx1 and RocF can be used in clinical screening of highly pathogenic <jats:italic>H. pylori<\/jats:italic> and the pathogenesis of <jats:italic>trx1<\/jats:italic> high expressing <jats:italic>H. pylori<\/jats:italic> remain still unknown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>We investigated the expression level of <jats:italic>H. pylori trx1<\/jats:italic> and <jats:italic>H. pylori rocF<\/jats:italic> in human gastric antrum tissues using reverse transcription and quantitative real\u2010time PCR (RT\u2010qPCR) and clarified the clinical application value of <jats:italic>trx1<\/jats:italic> and <jats:italic>rocF<\/jats:italic> for screening highly pathogenic <jats:italic>H. pylori<\/jats:italic>. The pathogenic mechanism of Trx1 were further explored by RNA\u2010seq of GES\u20101 cells co\u2010cultured with <jats:italic>trx1<\/jats:italic> high or low expressing <jats:italic>H. pylori<\/jats:italic>. Differentially expressed genes and signaling pathways were validated by RT\u2010qPCR, Enzyme\u2010linked immunosorbent assay (ELISA), western blot, immunohistochemistry and immunofluorescence. We also assessed the adherence of <jats:italic>trx1<\/jats:italic> high and low expressing <jats:italic>H. pylori<\/jats:italic> to GES\u20101 cells.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We found that <jats:italic>H. pylori trx1<\/jats:italic> and <jats:italic>H. pylori rocF<\/jats:italic> were more significantly expressed in the gastric cancer and peptic ulcer group than that in the gastritis group and the parallel diagnosis of <jats:italic>H. pylori trx1<\/jats:italic> and <jats:italic>H. pylori rocF<\/jats:italic> had high sensitivity. The <jats:italic>trx1<\/jats:italic> high expressing <jats:italic>H. pylori<\/jats:italic> had stronger adhesion ability to GES\u20101 cells and upregulated the interleukin (IL) 23A\/nuclear factor \u03baappaB (NF\u2010\u03baB)\/IL17A, IL6, IL8 pathway.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:italic>H. pylori trx1<\/jats:italic> and <jats:italic>H. pylori rocF<\/jats:italic> can be used in clinical screening of highly pathogenic <jats:italic>H. pylori<\/jats:italic> and predicting the outcome of <jats:italic>H. pylori<\/jats:italic> infection. The <jats:italic>trx1<\/jats:italic> high expressing <jats:italic>H. pylori<\/jats:italic> has stronger adhesion capacity and promotes the development of gastric diseases by upregulating the activation of NF\u2010\u03baB signaling pathway.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.13072","type":"journal-article","created":{"date-parts":[[2024,4,30]],"date-time":"2024-04-30T08:01:26Z","timestamp":1714464086000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["<i>Helicobacter pylori<\/i> with <i>trx1<\/i> high expression promotes gastric diseases via upregulating the <scp>IL23A<\/scp>\/<scp>NF\u2010\u03baB<\/scp>\/<scp>IL8<\/scp> pathway"],"prefix":"10.1111","volume":"29","author":[{"given":"Xin","family":"Guan","sequence":"first","affiliation":[{"name":"Beijing Key Laboratory for Helicobacter pylori Infection and Upper Gastrointestinal Diseases, Department of Gastroenterology Peking University Third Hospital  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