{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,31]],"date-time":"2026-08-31T18:51:09Z","timestamp":1788202269245,"version":"build-2803163510"},"reference-count":24,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T00:00:00Z","timestamp":1743984000000},"content-version":"vor","delay-in-days":37,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2025,3]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>In our center, RT\u2010PCR was integrated as a routine method to diagnose <jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> due to its higher availability after COVID\u201019 pandemics. The objective of this study was to assess the feasibility and performance of systematically performed RT\u2010PCR for <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> detection and clarithromycin (CLA) resistance in a real\u2010life clinical practice.<\/jats:p><\/jats:sec><jats:sec><jats:title>Materials and Methods<\/jats:title><jats:p>One hundred consecutive patients underwent an upper digestive endoscopy during which at least four biopsies (two from the antrum and two from the corpus) were obtained for RT\u2010PCR and culture with antibiogram and four additional biopsies for histology. The results of <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> detection were compared among RT\u2010PCR, histology, and bacterial culture, and the results of CLA susceptibility were compared between culture\u2010based antibiogram and RT\u2010PCR.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Out of 100 patients, 64 were positive for <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> by RT\u2010PCR, 66 by histology, and 53 by culture, with no statistically significant difference among the three methods (<jats:italic>p<\/jats:italic>\u2009&gt;\u20090.05). CLA resistance was found in 8 out of 45 patients (17.7%) by culture and in 12 out of 64 patients (18.7%) by PCR. In 8 <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content>\u2010positive patients by culture, the antibiogram could not be realized due to lack of viability of the strains. In one patient, after a double checking, discrepant results were observed, requiring a complementary molecular analysis by the French National Reference Center for Helicobacters, which confirmed the existence of a double population of <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> strains within biopsies, with and without CLA resistance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Our study demonstrates that in real\u2010life clinical practice, RT\u2010PCR is feasible and comparable in the ability to detect <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> and its resistance to CLA to bacterial culture with antibiogram and histology. Given its rapidity and limited dependence on the operator's interpretation, it appears preferable to the other methods.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.70031","type":"journal-article","created":{"date-parts":[[2025,4,7]],"date-time":"2025-04-07T19:23:50Z","timestamp":1744053830000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Real\u2010Time <scp>PCR<\/scp><i>Helicobacter pylori<\/i> Test in Comparison With Culture and Histology for <i>Helicobacter pylori<\/i> Detection and Identification of Resistance to Clarithromycin: A Single\u2010Center Real\u2010Life Study"],"prefix":"10.1111","volume":"30","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3551-1006","authenticated-orcid":false,"given":"Kateryna","family":"Priadko","sequence":"first","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, IMAD, Hepato\u2010Gastroenterology &amp; Digestive Oncology  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sophie\u2010Anne","family":"Gibaud","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Microbiological Control University Hospital of Nantes  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Amaury","family":"Druet","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, IMAD, Hepato\u2010Gastroenterology &amp; Digestive Oncology  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Louise","family":"Galmiche","sequence":"additional","affiliation":[{"name":"Department of Pathology University Hospital of Nantes  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francis","family":"Megraud","sequence":"additional","affiliation":[{"name":"INSERM U1312 BRIC, University of Bordeaux  Bordeaux France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"St\u00e9phane","family":"Corvec","sequence":"additional","affiliation":[{"name":"Department of Bacteriology and Microbiological Control University Hospital of Nantes  Nantes France"},{"name":"INSERM, Immunology and New Concepts in ImmunoTherapy, INCIT, UMR 1302 Nantes Universit\u00e9  Nantes France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tamara","family":"Matysiak\u2010Budnik","sequence":"additional","affiliation":[{"name":"Nantes Universit\u00e9, CHU Nantes, IMAD, Hepato\u2010Gastroenterology &amp; 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