{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,31]],"date-time":"2026-07-31T23:28:54Z","timestamp":1785540534602,"version":"3.56.0"},"reference-count":31,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T00:00:00Z","timestamp":1748131200000},"content-version":"vor","delay-in-days":24,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001843","name":"Science and Engineering Research Board","doi-asserted-by":"publisher","award":["EMR\/2017\/001637"],"award-info":[{"award-number":["EMR\/2017\/001637"]}],"id":[{"id":"10.13039\/501100001843","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001411","name":"Indian Council of Medical Research","doi-asserted-by":"publisher","award":["BMI\/12 (82)\/2021"],"award-info":[{"award-number":["BMI\/12 (82)\/2021"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001409","name":"Department of Science and Technology, Ministry of Science and Technology, India","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001409","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Helicobacter"],"published-print":{"date-parts":[[2025,5]]},"abstract":"<jats:title>ABSTRACT<\/jats:title><jats:sec><jats:title>Background<\/jats:title><jats:p>Gastric cancer (GC) is a leading cause of cancer\u2010related mortality worldwide, with <jats:styled-content style=\"fixed-case\"><jats:italic>Helicobacter pylori<\/jats:italic><\/jats:styled-content> (<jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content>) infection recognized as a significant risk factor. <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> infects approximately 50% of the global population, contributing to chronic gastritis, peptic ulcers, and the development of GC. The oncoprotein Gankyrin (PSMD10) has been implicated in various human cancers, including hepatocellular carcinoma, gastric cancer, and lung cancer, by modulating autophagy and inflammatory pathways.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>In this study, we explored the role of Gankyrin in <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content>\u2010induced gastric tumorigenesis via a Swiss albino mouse xenograft model. Mice were subcutaneously injected with <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content>\u2010infected AGS cells with or without Gankyrin knockdown.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We assessed tumor growth and inflammatory markers (TNF\u2010\u03b1 and IL\u20106) levels and Gankyrin's downstream signaling molecules (p53, pRb, and NF\u2010\u03baB). Our results demonstrated that Gankyrin knockdown significantly decreased tumor formation in Swiss albino mice engrafted with <jats:italic>H. Pylori<\/jats:italic>\u2010infected AGS cells. Notably, treatment with cyclosporine A significantly decreased the expression of TNF\u2010\u03b1 in all the AGS\u2010engrafted mice except the PBS group. Moreover, our results show that the downregulation of Gankyrin significantly elevated the expression of NF\u2010\u03baB, pRb, and p53.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings suggest that Gankyrin plays a crucial role in <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content>\u2010mediated GC progression by modulating inflammatory and tumor suppressor pathways. Targeting Gankyrin could provide a therapeutic strategy to mitigate the development of GC associated with <jats:styled-content style=\"fixed-case\"><jats:italic>H. pylori<\/jats:italic><\/jats:styled-content> infection.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hel.70046","type":"journal-article","created":{"date-parts":[[2025,5,25]],"date-time":"2025-05-25T23:48:27Z","timestamp":1748216907000},"update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":3,"title":["Gankyrin Inhibition Can Control <scp><i>Helicobacter pylori<\/i><\/scp> Generated Gastric Cancer Using In\u00a0Vivo Xenograft Models"],"prefix":"10.1111","volume":"30","author":[{"given":"Dharmendra","family":"Kashyap","sequence":"first","affiliation":[{"name":"Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore  Indore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pranit Hemant","family":"Bagde","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore  Indore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1388-6221","authenticated-orcid":false,"given":"Siddharth","family":"Singh","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore  Indore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nidhi","family":"Varshney","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore  Indore India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tarun Prakash","family":"Verma","sequence":"additional","affiliation":[{"name":"Department of Biosciences and Biomedical Engineering Indian Institute of Technology Indore  Indore 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