{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,28]],"date-time":"2025-10-28T02:42:51Z","timestamp":1761619371414},"reference-count":42,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2012,12,18]],"date-time":"2012-12-18T00:00:00Z","timestamp":1355788800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2013,7]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Liver fibrosis results in a disproportion of the hepatic composition and architecture, characterized by a progressive accumulation of fibrillar proteins at the liver parenchyma. Modulated\u2010differential scanning calorimetry (m<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>) is an experimental methodology able to determine the specific thermal signature from any biological substance, based on the variation in heat flow and heat capacity. As these physicochemical properties are directly influenced by compositional and structural changes, we decided to study the thermal behavior of the liver during fibrosis using m<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Liver fibrosis was induced in rats by bile duct ligation or carbon tetrachloride administration. Degree of liver fibrosis was determined by histological examination using the <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content>asson\u2010trichrome stain, accompanied by hepatic expression of \u03b1\u2010smooth muscle actin. The thermal analysis was performed in a modulated\u2010differential scanning calorimeter using 20\u2009mg of fresh liver mass.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The liver showed a characteristic thermal signature in control animals, which progressively differed among mild (<jats:styled-content style=\"fixed-case\">F1<\/jats:styled-content>), moderate (<jats:styled-content style=\"fixed-case\">F2<\/jats:styled-content>) and advanced (<jats:styled-content style=\"fixed-case\">F3<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">F4<\/jats:styled-content>) liver fibrosis. For heat flow, the hepatic thermal signature from <jats:styled-content style=\"fixed-case\">F3<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">F4<\/jats:styled-content> rats exhibited significant differences when compared with <jats:styled-content style=\"fixed-case\">F1<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">F2<\/jats:styled-content> and controls. In terms of heat capacity, liver specimens provided a specific thermal signature for each stage of disease, characterized by a transition temperature onset at 95\u00b0C for controls, whereas in <jats:styled-content style=\"fixed-case\">F1<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">F2<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">F3<\/jats:styled-content>\u2013<jats:styled-content style=\"fixed-case\">F4<\/jats:styled-content> animals this temperature significantly decreased to 93\u00b0C, 84\u00b0C and 75\u00b0C, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Because the liver shows a differential thermal signature according to the degree of fibrosis, m<jats:styled-content style=\"fixed-case\">DSC<\/jats:styled-content> could be a novel tool in the study of liver fibrosis progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12026","type":"journal-article","created":{"date-parts":[[2012,12,18]],"date-time":"2012-12-18T20:24:51Z","timestamp":1355862291000},"page":"785-794","source":"Crossref","is-referenced-by-count":5,"title":["Liver exhibits thermal variations according to the stage of fibrosis progression: A novel use of modulated\u2010differential scanning calorimetry for research in hepatology"],"prefix":"10.1111","volume":"43","author":[{"given":"Galileo","family":"Escobedo","sequence":"first","affiliation":[{"name":"Unit of Experimental Medicine General Hospital of Mexico  M\u00e9xico D.F. 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