{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,15]],"date-time":"2026-05-15T05:01:36Z","timestamp":1778821296768,"version":"3.51.4"},"reference-count":32,"publisher":"Wiley","issue":"8","license":[{"start":{"date-parts":[[2013,7,21]],"date-time":"2013-07-21T00:00:00Z","timestamp":1374364800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,8]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>For chronic hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> virus (<jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>) infection, the effects of current therapies are limited. <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> interference of virus\u2010specific genes has emerged as a potential antiviral mechanism. However, a suitable delivery vector is still to be developed. We studied a novel vector transferring <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> targeting hepatic cells <jats:italic>in vivo<\/jats:italic> and <jats:italic>in vitro<\/jats:italic> in order to find a new way to cure <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content>\u2010related live diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>The <jats:styled-content style=\"fixed-case\">preS1\u20109Arg<\/jats:styled-content> ligand was used to deliver <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> to <jats:styled-content style=\"fixed-case\">HepG<\/jats:styled-content>2 and to <jats:styled-content style=\"fixed-case\">HepG<\/jats:styled-content>2 2.2.15 cells. To validate the antiviral efficacy <jats:italic>in vivo<\/jats:italic>, a <jats:styled-content style=\"fixed-case\">HBV<\/jats:styled-content> viremic animal model was established by s.c. inoculation of <jats:styled-content style=\"fixed-case\">HepG<\/jats:styled-content>2 2.2.15 tumor cells in nude mice. The minimal retardation effect on the migration of <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> was detected by gel electrophoresis to confirm the combination and the optimal ratio. Hepatitis <jats:styled-content style=\"fixed-case\">B<\/jats:styled-content> surface antigen (<jats:styled-content style=\"fixed-case\">HBsAg<\/jats:styled-content>) levels were detected by semiquantitatively enzyme\u2010linked immunosorbent assay <jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels were quantified with quantitative real\u2010time polymerase chain reaction and protein levels were determined with immunoblots and immunohistochemistry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">PreS1\u20109Arg<\/jats:styled-content> peptide strongly combined and transferred <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> into <jats:styled-content style=\"fixed-case\">HepG<\/jats:styled-content>2 cells. <jats:styled-content style=\"fixed-case\">PreS1\u20109Arg<\/jats:styled-content>\u2010<jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> molecular conjugate effectively reduced the production of <jats:styled-content style=\"fixed-case\">HBsAg<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">HBV DNA<\/jats:styled-content> without liver toxicity <jats:italic>in vitro<\/jats:italic> and <jats:italic>in vivo<\/jats:italic>.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The results indicated that p<jats:styled-content style=\"fixed-case\">reS1\u20109Arg<\/jats:styled-content> may be a potential novel vector to deliver <jats:styled-content style=\"fixed-case\">siRNA<\/jats:styled-content> targeting liver cells.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12189","type":"journal-article","created":{"date-parts":[[2013,6,25]],"date-time":"2013-06-25T12:48:13Z","timestamp":1372164493000},"page":"897-906","source":"Crossref","is-referenced-by-count":13,"title":["Targeted delivery of si<scp>RNA<\/scp> against hepatitis <scp>B<\/scp> virus by pre<scp>S1<\/scp> peptide molecular ligand"],"prefix":"10.1111","volume":"44","author":[{"given":"Wenjuan","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine the Second Hospital Affiliated to Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the Second Hospital Affiliated to Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Yi","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the Second Hospital Affiliated to Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sufen","family":"Zhu","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the Second Hospital Affiliated to Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Weixian","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine the Second Hospital Affiliated to Chongqing Medical University  Chongqing China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,7,21]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.2188\/jea.JE20100190"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2006.05.022"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/ecam\/nem180"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.5812\/hepatmon.4294"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/gt.2008.12"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.5732\/cjc.012.10280"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1389"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01270-06"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.1890370208"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1021\/bi002443l"},{"key":"e_1_2_6_12_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M007540200"},{"key":"e_1_2_6_13_1","unstructured":"FedericaS MayC MorrisFet\u2009al.Peptide carrier for delivering siRNA into mammalian cells.2009: US: Patent7514530."},{"key":"e_1_2_6_14_1","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.06707-11"},{"key":"e_1_2_6_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1122"},{"key":"e_1_2_6_16_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0703778104"},{"key":"e_1_2_6_17_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp800157x"},{"key":"e_1_2_6_18_1","doi-asserted-by":"publisher","DOI":"10.1089\/hum.2010.083"},{"key":"e_1_2_6_19_1","doi-asserted-by":"publisher","DOI":"10.3389\/fgene.2012.00154"},{"key":"e_1_2_6_20_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.mt.6300168"},{"key":"e_1_2_6_21_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt1396"},{"key":"e_1_2_6_22_1","doi-asserted-by":"publisher","DOI":"10.1021\/mp3006006"},{"key":"e_1_2_6_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.1807"},{"key":"e_1_2_6_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jconrel.2012.08.030"},{"key":"e_1_2_6_25_1","doi-asserted-by":"publisher","DOI":"10.1038\/sj.gt.3302266"},{"key":"e_1_2_6_26_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.26181"},{"key":"e_1_2_6_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.biocel.2004.06.015"},{"key":"e_1_2_6_28_1","doi-asserted-by":"publisher","DOI":"10.1021\/jz100248c"},{"key":"e_1_2_6_29_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.14.3490"},{"key":"e_1_2_6_30_1","doi-asserted-by":"publisher","DOI":"10.2147\/IJN.S31365"},{"key":"e_1_2_6_31_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0025096"},{"key":"e_1_2_6_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1386-6532(05)80011-3"},{"key":"e_1_2_6_33_1","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260115"}],"container-title":["Hepatology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhepr.12189","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hepr.12189","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T23:17:53Z","timestamp":1696288673000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hepr.12189"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,7,21]]},"references-count":32,"journal-issue":{"issue":"8","published-print":{"date-parts":[[2014,8]]}},"alternative-id":["10.1111\/hepr.12189"],"URL":"https:\/\/doi.org\/10.1111\/hepr.12189","archive":["Portico"],"relation":{},"ISSN":["1386-6346","1872-034X"],"issn-type":[{"value":"1386-6346","type":"print"},{"value":"1872-034X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,7,21]]}}}