{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,1]],"date-time":"2025-11-01T17:21:27Z","timestamp":1762017687310},"reference-count":38,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2013,8,15]],"date-time":"2013-08-15T00:00:00Z","timestamp":1376524800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,10]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>The aim of this study was to clarify the relationship between the expression of micro\u2010<jats:styled-content style=\"fixed-case\">RNAs<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content>) in peripheral blood mononuclear cells (<jats:styled-content style=\"fixed-case\">PBMCs<\/jats:styled-content>) and clinical presentation in patients with primary biliary cirrhosis (<jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>This study involved 58 patients with <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>, patients with control diseases including 25 patients with autoimmune hepatitis (<jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content>), six patients with <jats:styled-content style=\"fixed-case\">PBC\u2010AIH<\/jats:styled-content> overlap syndrome, 23 patients with systemic lupus erythematosus (<jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content>), and 30 healthy controls. After <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content> was extracted from <jats:styled-content style=\"fixed-case\">PBMCs<\/jats:styled-content>, the expressions of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u201026a, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010328, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010299\u20105p, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010146a, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010155, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u201016, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010132 and let7a were quantified by real\u2010time <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content>. The relationships between all <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content> expressions and clinical test parameters were also examined.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>In <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>, the expressions of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010155 and <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010146a were significantly increased compared to those in healthy controls. For <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u201026a, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010299\u20105p, <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010328 and let\u20107a, although no significant difference was observed in expression between patients and healthy controls, expressions were significantly increased in <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> compared to those in <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content>. Expressions of <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010299\u20105p were significantly increased in <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content> patients resistant to treatment with ursodeoxycholic acid (<jats:italic>n<\/jats:italic>\u2009=\u200918) compared to those in healthy controls. In the evaluation of the relationship between <jats:styled-content style=\"fixed-case\">miRNA<\/jats:styled-content> expression and clinical test parameters, significant and positive correlations were found for <jats:styled-content style=\"fixed-case\">miR<\/jats:styled-content>\u2010299\u20105p with alkaline phosphatase, gamma\u2010glutamyl transpeptidase, total bilirubin and immunoglobulin <jats:styled-content style=\"fixed-case\">M<\/jats:styled-content> levels.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>The preset results suggest the existence of <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content> that exhibit disease\u2010specific increases in expression and <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content> closely correlated with clinical test values in <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>. Further analyses of these <jats:styled-content style=\"fixed-case\">miRNAs<\/jats:styled-content> may contribute to the elucidation of the pathology of <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12198","type":"journal-article","created":{"date-parts":[[2013,7,9]],"date-time":"2013-07-09T12:16:28Z","timestamp":1373372188000},"source":"Crossref","is-referenced-by-count":22,"title":["Expression of micro\u2010<scp>RNAs<\/scp> in peripheral blood mononuclear cells from primary biliary cirrhosis patients"],"prefix":"10.1111","volume":"44","author":[{"given":"Fumiko","family":"Katsushima","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Atsushi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Natsumi","family":"Sakamoto","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yukiko","family":"Kanno","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazumichi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hiromasa","family":"Ohira","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima 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