{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T18:25:34Z","timestamp":1791915934726,"version":"4.3.4"},"reference-count":42,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2014,1,7]],"date-time":"2014-01-07T00:00:00Z","timestamp":1389052800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,10]]},"abstract":"\n Aim<\/jats:title>\n Splenectomy improves hypersplenic thrombocytopenia in cirrhotic patients with hypersplenism. However, the long\u2010term influence of splenectomy has not been clarified. We examined whether splenectomy improved liver fibrosis and caused immunological changes.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n We collected liver and spleen specimens and peripheral blood (\n PB<\/jats:styled-content>\n ) from 26 patients with hepatitis\n C<\/jats:styled-content>\n virus\u2010related liver cirrhosis. An immunohistochemical examination of\n CD4<\/jats:styled-content>\n ,\n CD8<\/jats:styled-content>\n , forkhead box\n P3<\/jats:styled-content>\n , granzyme\n B<\/jats:styled-content>\n and transforming growth factor\u2010\u03b21, and\n M<\/jats:styled-content>\n asson\u2010trichrome stain were performed in spleen and liver tissues and in seven cases of follow\u2010up liver biopsy sections obtained after splenectomy. We obtained\n PB<\/jats:styled-content>\n before and at various intervals after splenectomy. We also examined the ratio of\n \n CD4\n +<\/jats:sup>\n <\/jats:styled-content>\n and\n \n CD8\n +<\/jats:sup>\n <\/jats:styled-content>\n lymphocytes in\n PB<\/jats:styled-content>\n using flow cytometry.\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n We observed improvements in liver fibrosis in four biopsy specimens obtained after splenectomy, in which fibrotic areas significantly decreased from 19.5% to 8.2% (\n P<\/jats:italic>\n \u2009<\u20090.05). Increases were also observed in the ratio of\n \n CD8\n +<\/jats:sup>\n <\/jats:styled-content>\n cells in\n PB<\/jats:styled-content>\n after splenectomy, which resulted in a significant decrease in the\n \n CD4\n +<\/jats:sup>\n <\/jats:styled-content>\n \/\n \n CD8\n +<\/jats:sup>\n <\/jats:styled-content>\n ratio (\n P<\/jats:italic>\n \u2009<\u20090.001). The carcinogenic rate in patients with a\n \n CD4\n +<\/jats:sup>\n <\/jats:styled-content>\n \u2009:\u2009\n \n CD8\n +<\/jats:sup>\n <\/jats:styled-content>\n ratio that decreased by more than 0.5 at 1\u2009month after splenectomy was significantly lower than that in patients with a ratio that decreased by less than 0.5 (\n P<\/jats:italic>\n \u2009<\u20090.05).\n <\/jats:p>\n <\/jats:sec>\n \n Conclusion<\/jats:title>\n Splenectomy may improve liver fibrosis and cause beneficial immunological changes in cirrhotic patients with hepatitis. Improvements in antitumor mechanisms can be also expected.<\/jats:p>\n <\/jats:sec>","DOI":"10.1111\/hepr.12234","type":"journal-article","created":{"date-parts":[[2013,9,3]],"date-time":"2013-09-03T08:38:09Z","timestamp":1378197489000},"source":"Crossref","is-referenced-by-count":64,"title":["Influence of splenectomy in patients with liver cirrhosis and hypersplenism"],"prefix":"10.1111","volume":"44","author":[{"given":"Yoriko","family":"Nomura","sequence":"first","affiliation":[{"name":"Department of Diagnostic Pathology Kurume University Hospital Kurume Japan"},{"name":"Department of Surgery Kurume University School of Medicine Kurume Japan"},{"name":"Department of Pathology Kurume University School of Medicine Kurume Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayoshi","family":"Kage","sequence":"additional","affiliation":[{"name":"Department of Diagnostic Pathology Kurume University Hospital Kurume 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