{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:59:07Z","timestamp":1765807147093},"reference-count":36,"publisher":"Wiley","issue":"11","license":[{"start":{"date-parts":[[2013,11,8]],"date-time":"2013-11-08T00:00:00Z","timestamp":1383868800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,10]]},"abstract":"Aim<\/jats:title>Tuberous sclerosis complex 2 (TSC2<\/jats:styled-content>), a tumor suppressor, may play an essential role in the regulation of cell growth and cell survival under energy stress conditions. In addition, TSC2<\/jats:styled-content> may act in concert with W<\/jats:styled-content>nt and energy signals by additional phosphorylation of glycogen synthase kinase 3\u03b2 (GSK3\u03b2<\/jats:styled-content>) to regulate cell growth. The expression levels and function of TSC2<\/jats:styled-content> and GSK3\u03b2<\/jats:styled-content> in hepatocellular carcinoma (HCC<\/jats:styled-content>) remain unclear.<\/jats:p><\/jats:sec>Methods<\/jats:title>The protein levels of TSC2<\/jats:styled-content> and GSK3\u03b2<\/jats:styled-content> were measured by immunohistochemistry in normal liver (n<\/jats:italic>\u2009=\u200920), HCC<\/jats:styled-content> (n<\/jats:italic>\u2009=\u200980) and pericancerous tissues (n<\/jats:italic>\u2009=\u200980). The correlations between TSC2<\/jats:styled-content>, and GSK3\u03b2<\/jats:styled-content> levels, clinicopathological features and patient survival were also analyzed.<\/jats:p><\/jats:sec>Results<\/jats:title>The protein levels of TSC2<\/jats:styled-content> and GSK3\u03b2<\/jats:styled-content> in HCC<\/jats:styled-content> tissues were significantly lower than that in normal liver tissues and pericancerous tissues (P<\/jats:italic>\u2009<\u20090.05). Decreased TSC2<\/jats:styled-content> and GSK3\u03b2<\/jats:styled-content> expression was found to be significantly correlated with advanced clinicopathological characteristics and poor prognosis. The results also showed that TSC2<\/jats:styled-content> protein levels were associated with GSK3\u03b2<\/jats:styled-content> expression in HCC<\/jats:styled-content> specimens.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>This is the first demonstration that the decreases in TSC2<\/jats:styled-content> and GSK3\u03b2<\/jats:styled-content> levels may be associated with vascular invasion, histological grade and tumor\u2013node\u2013metastasis classification.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12256","type":"journal-article","created":{"date-parts":[[2013,10,12]],"date-time":"2013-10-12T01:58:00Z","timestamp":1381543080000},"page":"1142-1150","source":"Crossref","is-referenced-by-count":17,"title":["Correlation between tuberous sclerosis complex 2 and glycogen synthase kinase 3 beta levels, and outcomes of patients with hepatocellular carcinoma treated by hepatectomy"],"prefix":"10.1111","volume":"44","author":[{"given":"Ka\u2010Te","family":"Huang","sequence":"first","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yue\u2010Han","family":"Huang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Peng","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bin","family":"He","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhen\u2010Kun","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xia","family":"Yu","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jian\u2010Ou","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Pathology The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Qi\u2010Yu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hong\u2010Qi","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yun\u2010Feng","family":"Shan","sequence":"additional","affiliation":[{"name":"Department of Hepatobiliary Surgery The First Affiliated Hospital of Wenzhou Medical University Wenzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,11,8]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2012.03.066"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.3322\/caac.20107"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-011-2159-4"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1120985109"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cub.2005.10.050"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.05.045"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00432-003-0431-0"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-0973"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(03)00929-2"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejca.2005.03.023"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2407-8-163"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2006.06.055"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2011.12.129"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2012.224"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1097\/JTO.0b013e31820c5189"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.2010.218404"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-09-3403"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.24079"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1677\/JOE-09-0456"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M512722200"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10549-012-2229-8"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840360630"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bcp.2009.08.022"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.mrrev.2008.06.001"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1385\/NMM:7:4:287"},{"key":"e_1_2_5_27_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2008.08.018"},{"key":"e_1_2_5_28_1","first-page":"2531","article-title":"The role of PI3K\/mTOR inhibition in combination with sorafenib in hepatocellular carcinoma treatment","volume":"32","author":"Gedaly R","year":"2012","journal-title":"Anticancer Res"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1159\/000327565"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1158\/1078-0432.CCR-04-0941"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.08.008"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.ajkd.2011.10.013"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.1186\/1471-2350-12-29"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1038\/onc.2010.630"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1042\/BST0390466"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0511342103"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc2676"}],"container-title":["Hepatology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhepr.12256","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hepr.12256","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,3]],"date-time":"2023-10-03T15:27:18Z","timestamp":1696346838000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hepr.12256"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2013,11,8]]},"references-count":36,"journal-issue":{"issue":"11","published-print":{"date-parts":[[2014,10]]}},"alternative-id":["10.1111\/hepr.12256"],"URL":"https:\/\/doi.org\/10.1111\/hepr.12256","archive":["Portico"],"relation":{},"ISSN":["1386-6346","1872-034X"],"issn-type":[{"value":"1386-6346","type":"print"},{"value":"1872-034X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2013,11,8]]}}}