{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T23:41:28Z","timestamp":1782949288731,"version":"3.54.5"},"reference-count":30,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,1,27]],"date-time":"2014-01-27T00:00:00Z","timestamp":1390780800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,9]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To identify a novel autoantibody specific to autoimmune hepatitis (<jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content>) and to evaluate its clinical significance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Non\u2010nuclear component protein extracted from normal human liver cell <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content>yroh<jats:styled-content style=\"fixed-case\">NH<\/jats:styled-content>pes cultures that reacted with sera from <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> patients on a western blot was identified as an antigenic protein and subjected to <jats:styled-content style=\"fixed-case\">N<\/jats:styled-content>\u2010terminal amino acid analysis to identify phosphoenolpyruvate carboxykinase 2 (<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2). Enzyme\u2010linked immunoassay (<jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content>) for anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody was conducted on sera samples from patients with <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> (<jats:italic>n<\/jats:italic>\u2009=\u200942), primary biliary cirrhosis (<jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>; <jats:italic>n<\/jats:italic>\u2009=\u200948), non\u2010alcoholic steatohepatitis (<jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200941), chronic hepatitis <jats:styled-content style=\"fixed-case\">C<\/jats:styled-content> (<jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200920), drug\u2010induced liver injury (<jats:styled-content style=\"fixed-case\">DILI<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200910), systemic lupus erythematosus (<jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content>, <jats:italic>n<\/jats:italic>\u2009=\u200916) and on sera samples from healthy volunteers (<jats:italic>n<\/jats:italic>\u2009=\u200930). Clinical findings were compared for <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> patients testing positive and negative for anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">ELISA<\/jats:styled-content> findings showed that mean anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody titer in sera from <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> patients was significantly higher than in <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content>, <jats:styled-content style=\"fixed-case\">DILI<\/jats:styled-content> and <jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content> patients, as well as in healthy volunteers. Anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody was present in 50.0% (21\/42) of <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content>, 14.6% (7\/48) of <jats:styled-content style=\"fixed-case\">PBC<\/jats:styled-content>, 4.9% (2\/41) of <jats:styled-content style=\"fixed-case\">NASH<\/jats:styled-content>, and 10.0% (2\/20) of <jats:styled-content style=\"fixed-case\">CHC<\/jats:styled-content> patients, 0% (0\/10) of <jats:styled-content style=\"fixed-case\">DILI<\/jats:styled-content>, 12.5% (2\/16) of <jats:styled-content style=\"fixed-case\">SLE<\/jats:styled-content> and in 3.3% (1\/30) of healthy volunteers. The sensitivity, specificity and accuracy of using the detection of anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody in diagnosing <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> were 50.0%, 91.5% and 83.1%, respectively. None of the <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content> patients positive for anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 antibody showed characteristic clinical features.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Although further investigations into the clinical usefulness are necessary, anti\u2010<jats:styled-content style=\"fixed-case\">PCK<\/jats:styled-content>2 may have potential as a diagnostic marker for <jats:styled-content style=\"fixed-case\">AIH<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12276","type":"journal-article","created":{"date-parts":[[2013,11,18]],"date-time":"2013-11-18T13:45:54Z","timestamp":1384782354000},"page":"1019-1025","source":"Crossref","is-referenced-by-count":7,"title":["Anti\u2010phosphoenolpyruvate carboxykinase 2 antibody in patients with autoimmune hepatitis"],"prefix":"10.1111","volume":"44","author":[{"given":"Yukiko","family":"Kanno","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiroshi","family":"Watanabe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Atsushi","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kazumichi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hiromasa","family":"Ohira","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Rheumatology Fukushima Medical University  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2014,1,27]]},"reference":[{"key":"e_1_2_6_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S1665-2681(19)32081-2"},{"key":"e_1_2_6_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00904.x"},{"key":"e_1_2_6_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2011.00808.x"},{"key":"e_1_2_6_5_1","doi-asserted-by":"publisher","DOI":"10.1007\/s00535-011-0421-y"},{"key":"e_1_2_6_6_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1872-034X.2012.01105.x"},{"key":"e_1_2_6_7_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2010.04.053"},{"key":"e_1_2_6_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12072-010-9183-5"},{"key":"e_1_2_6_9_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMra050408"},{"key":"e_1_2_6_10_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.21807"},{"key":"e_1_2_6_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80297-9"},{"key":"e_1_2_6_12_1","first-page":"1261","article-title":"Primary biliary cirrhosis. 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