{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,7]],"date-time":"2026-10-07T15:42:46Z","timestamp":1791387766862,"version":"4.3.3"},"reference-count":35,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2014,2,17]],"date-time":"2014-02-17T00:00:00Z","timestamp":1392595200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,9]]},"abstract":"Aim<\/jats:title>Recent evidences indicate that hepatic steatosis suppresses autophagic proteolysis. The present study evaluated the correlation between autophagic function and cathepsin expression in the liver from patients with non\u2010alcoholic fatty liver disease (NAFLD<\/jats:styled-content>).<\/jats:p><\/jats:sec>Methods<\/jats:title>Liver biopsy specimens were obtained from patients with chronic liver diseases (chronic hepatitis C<\/jats:styled-content> [CHC<\/jats:styled-content>; n<\/jats:italic>\u2009=\u200920], chronic hepatitis B<\/jats:styled-content> [CHB<\/jats:styled-content>; n<\/jats:italic>\u2009=\u200916], primary biliary cirrhosis [PBC<\/jats:styled-content>; n<\/jats:italic>\u2009=\u200923], NAFLD<\/jats:styled-content> [n<\/jats:italic>\u2009=\u200922] and control [n<\/jats:italic>\u2009=\u200914]). The number of autophagic vesicles in hepatocytes was counted by using transmission electron microscopy. Expression of cathepsin B<\/jats:styled-content>, D<\/jats:styled-content>, L<\/jats:styled-content> and p62 in the liver section was analyzed by immunohistochemical staining. The histological severity of NAFLD<\/jats:styled-content> is assessed by NAFLD<\/jats:styled-content> activity score (NAS<\/jats:styled-content>).<\/jats:p><\/jats:sec>Results<\/jats:title>The number of autophagic vesicles in hepatocytes was significantly increased in both CHC<\/jats:styled-content> and NAFLD<\/jats:styled-content> groups, but not CHB<\/jats:styled-content> and PBC<\/jats:styled-content>, more than control. Although hepatocytes with aggregation of p62 were observed in less than 15% of CHC<\/jats:styled-content>, p62 aggregation was detected in approximately 65% of NAFLD<\/jats:styled-content>. Cathepsin B<\/jats:styled-content>, D<\/jats:styled-content> and L<\/jats:styled-content> expression was significantly suppressed in the liver from NAFLD<\/jats:styled-content> patients. Suppression of cathepsin B<\/jats:styled-content>, D<\/jats:styled-content> and L<\/jats:styled-content> expression was not observed in CHB<\/jats:styled-content>, CHC<\/jats:styled-content> and PBC<\/jats:styled-content>. In NAFLD<\/jats:styled-content> patients, p62 aggregation was correlated with serum alanine aminotransferase value and inflammatory activity by NAS<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>These results indicate that a decrease in hepatic cathepsin expression in NAFLD<\/jats:styled-content> is associated with autophagic dysfunction. Hepatic inflammation correlates with autophagic dysfunction in NAFLD<\/jats:styled-content>. These findings indicate that the suppression of autophagic proteolysis by hepatic steatosis is involved in the pathogenesis of NAFLD<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12282","type":"journal-article","created":{"date-parts":[[2013,12,3]],"date-time":"2013-12-03T02:15:03Z","timestamp":1386036903000},"page":"1026-1036","source":"Crossref","is-referenced-by-count":174,"title":["Abnormality of autophagic function and cathepsin expression in the liver from patients with non\u2010alcoholic fatty liver disease"],"prefix":"10.1111","volume":"44","author":[{"given":"Yuka","family":"Fukuo","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Juntendo University School of Medicine Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shunhei","family":"Yamashina","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology Juntendo University School of Medicine Tokyo 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