{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,25]],"date-time":"2026-04-25T11:20:26Z","timestamp":1777116026009,"version":"3.51.4"},"reference-count":37,"publisher":"Wiley","issue":"13","license":[{"start":{"date-parts":[[2014,2,28]],"date-time":"2014-02-28T00:00:00Z","timestamp":1393545600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2014,12]]},"abstract":"Aim<\/jats:title>Hepatic apoptosis is involved in the pathogenesis of immune\u2010mediated liver diseases such as autoimmune hepatitis (AIH<\/jats:styled-content>), primary biliary cirrhosis (PBC<\/jats:styled-content>) and primary sclerosing cholangitis (PSC<\/jats:styled-content>). The aim of our study was to quantify distinct markers of apoptosis in sera of patients with AIH<\/jats:styled-content>, PBC<\/jats:styled-content> and PSC<\/jats:styled-content>, and to evaluate correlation with markers of disease activity and prognosis.<\/jats:p><\/jats:sec>Methods<\/jats:title>Sera of patients with AIH<\/jats:styled-content>, PBC<\/jats:styled-content> and PSC<\/jats:styled-content>, and of healthy controls were collected and distinct cell death markers were quantified using a bead\u2010based multiplex enzyme linked immunosorbent assay (soluble intracellular adhesion molecule [sICAM<\/jats:styled-content>], macrophage migration inhibitory factor [MIF<\/jats:styled-content>], soluble F<\/jats:styled-content>as [sF<\/jats:styled-content>as], plasminogen activator inhibitor 1 [PAI<\/jats:styled-content>\u20101]) or single enzyme\u2010linked immunosorbent assays (DNAse<\/jats:styled-content>, M<\/jats:styled-content>30, M<\/jats:styled-content>65).<\/jats:p><\/jats:sec>Results<\/jats:title>In comparison with healthy controls, the apoptotic markers sFas<\/jats:styled-content>, sICAM<\/jats:styled-content> (only in PSC<\/jats:styled-content> patients), M<\/jats:styled-content>30 and the cell death marker M<\/jats:styled-content>65 were substantially elevated in sera of patients with immune\u2010mediated liver diseases, whereas DNAse<\/jats:styled-content> activity was reduced. Interestingly, patients with advanced PSC<\/jats:styled-content> presented with higher levels of sICAM<\/jats:styled-content>, M<\/jats:styled-content>30 and M<\/jats:styled-content>65 than patients with mild PSC<\/jats:styled-content>. Regression analysis revealed correlations between serum levels of sICAM<\/jats:styled-content>, M<\/jats:styled-content>30 and M<\/jats:styled-content>65 with the M<\/jats:styled-content>ayo R<\/jats:styled-content>isk S<\/jats:styled-content>core for PSC<\/jats:styled-content>, and of M<\/jats:styled-content>65 with the M<\/jats:styled-content>ayo R<\/jats:styled-content>isk S<\/jats:styled-content>core for PBC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>Concentrations of the serum markers of apoptosis sFas<\/jats:styled-content> and M<\/jats:styled-content>30 and of the marker of total cell death M<\/jats:styled-content>65 are elevated in patients with immune\u2010mediated liver diseases, whereas activity of DNAse<\/jats:styled-content> is reduced. In patients with PSC<\/jats:styled-content>, sICAM<\/jats:styled-content>, M<\/jats:styled-content>30 and M<\/jats:styled-content>65 may serve as indicators for disease activity and prognosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12304","type":"journal-article","created":{"date-parts":[[2014,1,23]],"date-time":"2014-01-23T12:25:47Z","timestamp":1390479947000},"page":"1286-1298","source":"Crossref","is-referenced-by-count":20,"title":["Soluble intracellular adhesion molecule, M<\/scp>30 and M<\/scp>65 as serum markers of disease activity and prognosis in cholestatic liver diseases"],"prefix":"10.1111","volume":"44","author":[{"given":"Gerald","family":"Denk","sequence":"first","affiliation":[{"name":"Department of Medicine II \u2013 Grosshadern University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ahmed\u2010Jawid","family":"Omary","sequence":"additional","affiliation":[{"name":"Department of Medicine II \u2013 Grosshadern University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian Paul","family":"Reiter","sequence":"additional","affiliation":[{"name":"Department of Medicine II \u2013 Grosshadern University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Simon","family":"Hohenester","sequence":"additional","affiliation":[{"name":"Department of Medicine II \u2013 Grosshadern University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ralf","family":"Wimmer","sequence":"additional","affiliation":[{"name":"Department of Medicine II \u2013 Grosshadern University of Munich Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Stefan","family":"Holdenrieder","sequence":"additional","affiliation":[{"name":"Institute for Clinical Chemistry and Clinical Pharmacology University Hospital Bonn Bonn Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Rust","sequence":"additional","affiliation":[{"name":"Department of Medicine I Krankenhaus Barmherzige Br\u00fcder Munich Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2014,2,28]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1586\/14737159.9.2.139"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrgastro.2011.69"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-2007-979469"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.gtc.2011.03.008"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.04.009"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-642-03503-6_7"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12016-008-8085-y"},{"key":"e_1_2_5_9_1","doi-asserted-by":"publisher","DOI":"10.1055\/s-0030-1267540"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1080\/10408360802485875"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.canlet.2013.04.013"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0168-8278(99)80297-9"},{"key":"e_1_2_5_13_1","first-page":"2047","article-title":"Apoptosis\u2010related biomarkers sFAS, MIF, ICAM\u20101 and PAI\u20101 in serum of breast cancer patients undergoing neoadjuvant chemotherapy","volume":"32","author":"Fersching DM","year":"2012","journal-title":"Anticancer Res"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0025-6196(11)64614-4"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840100102"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1159\/000299799"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1053\/j.gastro.2008.03.002"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0676.2001.021004272.x"},{"key":"e_1_2_5_19_1","first-page":"1274","article-title":"Immunohistochemical detection of Fas and apoptosis in type\u20101 autoimmune hepatitis","volume":"50","author":"Seki S","year":"2003","journal-title":"Hepatogastroenterology"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1034\/j.1600-0676.2000.020002129.x"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.2006.03293.x"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.3109\/08923970009016403"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2249.1994.tb06019.x"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.trsl.2006.11.010"},{"key":"e_1_2_5_25_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.510250332"},{"key":"e_1_2_5_26_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.214"},{"key":"e_1_2_5_27_1","doi-asserted-by":"crossref","first-page":"651","DOI":"10.1055\/s-0038-1646877","article-title":"Plasminogen activators and plasminogen activator inhibitors in liver deficiencies caused by chronic alcoholism or infectious hepatitis","volume":"62","author":"Tran\u2010Thang C","year":"1989","journal-title":"Thromb Haemost"},{"key":"e_1_2_5_28_1","doi-asserted-by":"publisher","DOI":"10.1136\/jcp.47.3.218"},{"key":"e_1_2_5_29_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajpgi.00107.2009"},{"key":"e_1_2_5_30_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.jhep.2009.02.025"},{"key":"e_1_2_5_31_1","doi-asserted-by":"publisher","DOI":"10.1002\/hep.1840160330"},{"key":"e_1_2_5_32_1","doi-asserted-by":"publisher","DOI":"10.1136\/gut.36.4.604"},{"key":"e_1_2_5_33_1","doi-asserted-by":"publisher","DOI":"10.3748\/wjg.v13.i6.837"},{"key":"e_1_2_5_34_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11695-008-9506-y"},{"key":"e_1_2_5_35_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood-2006-10-049817"},{"key":"e_1_2_5_36_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcla.20180"},{"key":"e_1_2_5_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.clinbiochem.2006.12.010"},{"key":"e_1_2_5_38_1","doi-asserted-by":"publisher","DOI":"10.1196\/annals.1368.043"}],"container-title":["Hepatology Research"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhepr.12304","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/hepr.12304","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,2]],"date-time":"2023-10-02T08:33:04Z","timestamp":1696235584000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/hepr.12304"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2014,2,28]]},"references-count":37,"journal-issue":{"issue":"13","published-print":{"date-parts":[[2014,12]]}},"alternative-id":["10.1111\/hepr.12304"],"URL":"https:\/\/doi.org\/10.1111\/hepr.12304","archive":["Portico"],"relation":{},"ISSN":["1386-6346","1872-034X"],"issn-type":[{"value":"1386-6346","type":"print"},{"value":"1872-034X","type":"electronic"}],"subject":[],"published":{"date-parts":[[2014,2,28]]}}}