{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,20]],"date-time":"2025-10-20T18:33:34Z","timestamp":1760985214082,"version":"3.37.3"},"reference-count":32,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2015,1,13]],"date-time":"2015-01-13T00:00:00Z","timestamp":1421107200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"crossref","award":["25220206","23590976"],"award-info":[{"award-number":["25220206","23590976"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2015,9]]},"abstract":"Aim<\/jats:title>Most of the modification of N<\/jats:styled-content><\/jats:italic>\u2010glycosylation reported in cancers including hepatocellular carcinoma (HCC<\/jats:styled-content>) were based on the examinations of a small number of patients or particular proteins. The aim of this study is to reveal changes in whole serum N<\/jats:styled-content><\/jats:italic>\u2010glycan profiles systematically during the process of hepatocarcinogenesis and to elucidate their clinical application.<\/jats:p><\/jats:sec>Methods<\/jats:title>We analyzed sera from 105 patients with chronic hepatitis\/liver cirrhosis (CH<\/jats:styled-content>\/LC<\/jats:styled-content>) and age\u2010\/sex\u2010matched healthy volunteers (HLT<\/jats:styled-content>), as well as from 114 patients with HCC<\/jats:styled-content>. Serum N<\/jats:styled-content><\/jats:italic>\u2010glycan profiles were measured comprehensively by a new, quantitative, high\u2010throughput method and compared with clinical parameters.<\/jats:p><\/jats:sec>Results<\/jats:title>The total amount of N<\/jats:styled-content><\/jats:italic>\u2010glycan expression was significantly higher in patients with CH<\/jats:styled-content>\/LC<\/jats:styled-content> than in HLT<\/jats:styled-content>; however, no differences were observed between CH<\/jats:styled-content>\/LC<\/jats:styled-content> and HCC<\/jats:styled-content> patients. In HCC<\/jats:styled-content> patients, multi\u2010antennary glycans with fucose residues, particularly m\/z<\/jats:italic> 3195, were increased compared with CH<\/jats:styled-content>\/LC<\/jats:styled-content> patients. The expression of m\/z<\/jats:italic> 3195 was high, especially in patients with a high number of intrahepatic lesions (>3), large tumor size (>3\u2009cm), macroscopic vascular invasion or metastasis. The ratio of pairs of glycans on the same path of the biosynthesis pathway (m\/z<\/jats:italic> 3195\/1914) showed a higher area under the receiver\u2013operator curve of 0.810 than any other single glycan to distinguish HCC<\/jats:styled-content> from CH<\/jats:styled-content>\/LC<\/jats:styled-content>.<\/jats:p><\/jats:sec>Conclusion<\/jats:title>We demonstrate the full spectrum of the alterations of serum N<\/jats:styled-content><\/jats:italic>\u2010glycans comprehensively in patients with liver disease, and elucidate the possible use of glycans as novel biomarkers of liver disease progression.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12441","type":"journal-article","created":{"date-parts":[[2014,12,12]],"date-time":"2014-12-12T12:34:36Z","timestamp":1418387676000},"page":"986-993","source":"Crossref","is-referenced-by-count":17,"title":["Alteration of N<\/scp><\/i>\u2010glycan profiles in patients with chronic hepatitis and hepatocellular carcinoma"],"prefix":"10.1111","volume":"45","author":[{"given":"Koji","family":"Miyahara","sequence":"first","affiliation":[{"name":"Department of Gastroenterology and Hepatology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kazuhiro","family":"Nouso","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"},{"name":"Department of Molecular Hepatology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chihiro","family":"Dohi","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences Okayama 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