{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,6]],"date-time":"2025-11-06T20:02:45Z","timestamp":1762459365896},"reference-count":52,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2016,6,1]],"date-time":"2016-06-01T00:00:00Z","timestamp":1464739200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81370554"],"award-info":[{"award-number":["81370554"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2017,3]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>It remains unclear whether intrahepatic angiogenesis increases portal hypertension (PH) in hepatitis B with cirrhosis. We aim to investigate the relationship between intrahepatic angiogenesis and PH in hepatitis B patients with cirrhosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Sixty hepatitis B patients with cirrhosis and 40 healthy subjects were included in this study. Angiogenesis markers vascular endothelial growth factor receptor\u20102 (VEGFR2), von Willebrand factor (vWF), and fibrosis marker \u03b1\u2010smooth muscle actin (\u03b1\u2010SMA) were observed by immunohistochemistry. Sirius Red staining was also used to determine liver fibrosis. Correlations between levels of intrahepatic angiogenesis and Child\u2013Pugh classes, liver fibrosis degree, and portal vein pressure were examined. We also analyzed the relationship between levels of intrahepatic angiogenesis and complications of PH, including esophageal varices (EV), ascites, and hypersplenism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Correlation was observed between the levels of VEGFR2 (r\u2009=\u20090.590, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), vWF (r\u2009=\u20090.524, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01) in tissue, and Child\u2013Pugh classes. Significant correlations were observed between levels of VEGFR2 and \u03b1\u2010SMA (r\u2009=\u20090.710, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), VEGFR2 and Sirius Red (r\u2009=\u20090.841, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), vWF and \u03b1\u2010SMA (r\u2009=\u20090.768, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), and vWF and Sirius Red (r\u2009=\u20090.825, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01). Patients with hepatic venous pressure gradient (HVPG) \u226512\u00a0mmHg showed higher levels of VEGFR2 and vWF expression compared to those with (HVPG) &lt;12\u00a0mmHg (2.60\u2009\u00b1\u20091.28% vs. 1.09\u2009\u00b1\u20090.73%; 5.85\u2009\u00b1\u20092.45% vs. 2.31\u2009\u00b1\u20091.34%, <jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), respectively. Moreover, complications of PH, including size of esophageal varices (<jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), presence of ascites (<jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01), and spleen volume (<jats:italic>P<\/jats:italic>\u2009&lt;\u00a00.01) were significantly affected by the levels of intrahepatic angiogenesis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>Intrahepatic angiogenesis increases PH in hepatitis B patients with cirrhosis. The study provides the potential ways to intervene in the progresses for therapeutic benefits in cirrhosis and PH.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.12732","type":"journal-article","created":{"date-parts":[[2016,4,27]],"date-time":"2016-04-27T14:18:29Z","timestamp":1461766709000},"source":"Crossref","is-referenced-by-count":7,"title":["Intrahepatic angiogenesis increases portal hypertension in hepatitis B patients with cirrhosis"],"prefix":"10.1111","volume":"47","author":[{"given":"Le","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Shandong Provincial Hospital Affiliated to Shandong University  Jinan Shandong China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinya","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Radiology Shandong Provincial Hospital Affiliated to Shandong University  Jinan Shandong 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