{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T16:31:55Z","timestamp":1780417915887,"version":"3.54.1"},"reference-count":29,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2018,1,10]],"date-time":"2018-01-10T00:00:00Z","timestamp":1515542400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The Research Program on Hepatitis","award":["JP17fk0210302h"],"award-info":[{"award-number":["JP17fk0210302h"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2018,5]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Patients with resolved hepatitis B virus (HBV) infection following hematopoietic stem cell transplantation (HSCT) are potentially at high risk of HBV reactivation. Although antiviral drug therapy is recommended when HBV DNA reappears in the serum, drug efficacy after HBV reactivation remains unclear.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Host immune response against HBV was investigated by immunological analyses at 12\u00a0months after entecavir (ETV) treatment in six HSCT\u2010treated and five non\u2010HSCT\u2010treated patients with HBV reactivation, and 18 patients with chronic hepatitis B (CHB). Peripheral HBV\u2010specific CD8<jats:sup>+<\/jats:sup> T cells were analyzed for total numbers by flow cytometry and tetramer staining, as was intracellular \u03b3\u2010interferon (IFN\u2010\u03b3) production and CD107a expression in response to HBV peptides. Interleukin\u201010 (IL\u201010)\u2010expressing CD19<jats:sup>+<\/jats:sup> B\u2010cell count and serum inflammatory cytokine levels were also analyzed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Serum HBV DNA was detectable in HSCT\u2010treated patients with HBV reactivation at 12\u00a0months compared with other groups, indicating insufficient ETV efficacy against HBV. The HBV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell counts in HSCT\u2010treated patients with HBV reactivation were significantly lower compared with those in non\u2010HSCT patients. Additionally, IFN\u2010\u03b3 production and CD107a expression by CD8<jats:sup>+<\/jats:sup> T cells after incubation with HBV peptides was significantly reduced in HSCT\u2010treated compared with CHB patients at 12\u00a0months after ETV treatment. Conversely, HSCT\u2010treated patient serum IL\u201010 levels were significantly elevated compared with those in non\u2010HSCT patients. Finally, IL\u201010\u2010producing CD19<jats:sup>+<\/jats:sup> B\u2010cell counts were increased in HSCT\u2010treated compared with CHB patients.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>After HBV reactivation, ETV efficacy was impaired in HSCT\u2010treated patients as evidenced by low HBV\u2010specific CD8<jats:sup>+<\/jats:sup> T\u2010cell counts and high B\u2010cell IL\u201010 production.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13044","type":"journal-article","created":{"date-parts":[[2017,12,13]],"date-time":"2017-12-13T07:22:58Z","timestamp":1513149778000},"page":"469-478","source":"Crossref","is-referenced-by-count":6,"title":["Reduced therapeutic effect of antiviral drugs in patients with hepatitis B virus reactivation after hematopoietic stem cell transplantation"],"prefix":"10.1111","volume":"48","author":[{"given":"Masamichi","family":"Kimura","sequence":"first","affiliation":[{"name":"Division of 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