{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,20]],"date-time":"2026-09-20T17:24:55Z","timestamp":1789925095732,"version":"4.0.1"},"reference-count":35,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2018,9,4]],"date-time":"2018-09-04T00:00:00Z","timestamp":1536019200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"name":"The young researcher French Interregional Clinical Research and Innovation grant"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2019,1]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to evaluate the accuracy of the dosage of calprotectin in ascitic fluid (AF) using the Quantum Blue assay, for the prompt diagnosis of spontaneous bacterial peritonitis (SBP).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We prospectively collected 236 AF samples from 119 cirrhotic patients hospitalized in two French centers between May 2016 and May 2017. Bloody and chylous\/cloudy AF, and secondary peritonitis were excluded. SBP was diagnosed if neutrophils in AF were &gt;250\/mm<jats:sup>3<\/jats:sup> using standard cytology. The Quantum Blue Reader selectively measured the calprotectin antigen (MRP8\/14) in 12\u00a0min within the measurable range from 0.18 to 1.80\u00a0\u03bcg\/mL; values outside this range were registered as 0.17 and 1.81\u00a0\u03bcg\/mL.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>A total of 36 AF were considered as SBP (15.2%). SBP had higher median levels of calprotectin than non\u2010SBP (1.81 vs. 0.25\u00a0\u03bcg\/mL, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001). Calprotectin levels were positively correlated with neutrophils in AF (<jats:italic>r<\/jats:italic>\u2009=\u20090.57, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001) and C\u2010reactive protein (<jats:italic>r<\/jats:italic>\u2009=\u20090.43, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.001), but not with the Child\u2013Pugh and Model for End\u2010Stage Liver Disease scores. The optimal threshold of calprotectin to diagnose SBP was set at 1.51\u00a0\u03bcg\/mL (80th percentile of calprotectin), yielding sensitivity, specificity, and positive and negative predictive values of 86.1%, 92.0%, 65.9%, and 97.3%, respectively. Only one asymptomatic patient with SBP had a low calprotectin level, but a high serum C\u2010reactive protein level that strongly suggested an ongoing infection. We also showed that intraclass correlation coefficients for inter\u2010 and intra\u2010observer agreement were excellent, with 0.95 and 0.89, respectively.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The dosage of calprotectin in AF using the Quantum Blue assay is a rapid and reliable method of ruling out SBP in hospitalized cirrhotic patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13239","type":"journal-article","created":{"date-parts":[[2018,8,7]],"date-time":"2018-08-07T12:43:57Z","timestamp":1533645837000},"page":"72-81","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":16,"title":["Accuracy of calprotectin using the Quantum Blue Reader for the diagnosis of spontaneous bacterial peritonitis in liver 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