{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,25]],"date-time":"2026-09-25T02:16:25Z","timestamp":1790302585393,"version":"4.1.0"},"reference-count":40,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,2,7]],"date-time":"2019-02-07T00:00:00Z","timestamp":1549497600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81401577"],"award-info":[{"award-number":["81401577"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81571877"],"award-info":[{"award-number":["81571877"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2019,3]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>We aimed to evaluate effects of RC\u20103095 on mice with hepatic ischemia followed by reperfusion (I\/R) injury and further explore the possible underlying mechanism.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Mice were subjected to partial hepatic ischemia for 60\u00a0min followed by different durations of reperfusion. Levels of gastrin\u2010releasing peptide (GRP) and GRP receptor (GRPR) in the blood and liver were detected by enzyme\u2010linked immunosorbent assay (ELISA) or western blotting (WB) after 3, 6, 12, or 24\u00a0h of reperfusion. RC\u20103095 or normal saline (control) was given i.p. at the time of reperfusion. Expressions of tumor necrosis factor (TNF)\u2010\u03b1, interleukin (IL)\u20101\u03b2, IL\u20106, and IL\u201010 in blood and liver samples were examined with ELISA. Neutrophil influx into the liver was assessed by flow cytometry and myeloperoxidase assay. Hematoxylin\u2013eosin staining of the liver and terminal deoxynucleotidyl transferase mediated dUTP\u2010biotin nick end labeling assay were used to determine hepatic injury and hepatocellular necrosis. Activation of nuclear factor (NF)\u2010\u03baB and p38\/extracellular regulated protein kinase (ERK) mitogen activated protein kinase (MAPK) was investigated with WB.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of GRP was upregulated within 3\u00a0h after reperfusion and remained elevated for up to 24\u00a0h in the liver, whereas GRPR was also upregulated after 3 or 6\u00a0h of reperfusion, but returned to baseline levels within 24\u00a0h. RC\u20103095 significantly reduced the inflammatory hepatic injury, liver neutrophil accumulation, and hepatocellular apoptosis, probably by inhibiting activation of NF\u2010\u03baB or p38\/ERK MAPK.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p>These findings supported that GRP\u2013GRPR played an important role in hepatic I\/R injury, and RC\u20103095 ameliorated liver damage by suppressing the inflammatory response and hepatocellular necrosis.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13315","type":"journal-article","created":{"date-parts":[[2019,1,18]],"date-time":"2019-01-18T07:29:17Z","timestamp":1547796557000},"page":"247-255","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Protective effects of gastrin\u2010releasing peptide receptor antagonist RC\u20103095 in an animal model of hepatic ischemia\/reperfusion 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