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Hepatic oxidative damage was assessed by the expression of oxidation and antioxidant genes, and the correlation between oxidative damage and BA incidence, liver inflammation, and fibrosis was evaluated. Moreover, 8\u2010hydroxyguanine (8\u2010OHdG), mitochondrial DNA (mtDNA) copy number, and mitochondrial gene sequences were determined to evaluate oxidative mtDNA damage in BA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The expression of oxidation gene cytochrome b\u2010245 beta chain (<jats:italic>CYBB<\/jats:italic>) in BA was significantly increased and patients with a higher <jats:italic>CYBB<\/jats:italic> expression had the higher risk of BA incidence, liver inflammation, and cirrhosis. However, the expression of antioxidant genes was significantly decreased, and glutathione S\u2010transferase alpha 1 (<jats:italic>GSTA1<\/jats:italic>) negatively correlated with BA incidence and cirrhosis. When <jats:italic>GSTA1<\/jats:italic> mRNA expression was &lt;0.5487, the sensitivity was 80.85% and the specificity was 80% for BA diagnosis. Moreover, 8\u2010OHdG was increased, whereas mtDNA copy number was significantly decreased in BA. Using mitochondrial gene sequencing, 10 mutation sites were identified, and one family showed a maternal inheritance in genetic loci 15\u2009326.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>In BA, oxidative damage positively correlated with BA incidence, liver inflammation, and cirrhosis. <jats:italic>GSTA1<\/jats:italic> could be a novel diagnostic indicator. Genetic loci 15\u2009326 could be a maternal genetic mutation site. Taken together, antioxidation therapy after Kasai surgery might have great potential in relieving liver inflammation and fibrosis in BA patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13324","type":"journal-article","created":{"date-parts":[[2019,2,27]],"date-time":"2019-02-27T15:33:14Z","timestamp":1551281594000},"page":"695-704","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["Correlation between hepatic oxidative damage and clinical severity and mitochondrial gene sequencing results in biliary atresia"],"prefix":"10.1111","volume":"49","author":[{"given":"Junfeng","family":"Wang","sequence":"first","affiliation":[{"name":"Department of Pediatric Surgery, Children's Hospital of Fudan University  Shanghai China"},{"name":"Key Laboratory of Birth Defects, Children's Hospital of Fudan University Shanghai 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