{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:52:51Z","timestamp":1762102371201,"version":"3.40.5"},"reference-count":27,"publisher":"Wiley","issue":"9","license":[{"start":{"date-parts":[[2019,6,3]],"date-time":"2019-06-03T00:00:00Z","timestamp":1559520000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2019,9]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>A significant concern for autoimmune hepatitis (AIH) patients is diagnostic specificity. Delayed treatment due to delayed diagnosis leads to poor survival. We recently reported that chemokine C\u2010C receptor 7 (CCR7)<jats:sup>\u2212<\/jats:sup>\/programmed cell death\u20101 (PD\u20101)<jats:sup>+<\/jats:sup> follicular helper T (Tfh) cells could be involved in AIH pathogenesis. We hypothesized that Tfh cell frequencies might contribute to AIH diagnosis.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Peripheral blood was collected from 12 patients with AIH from April 2013 to March 2016, as well as 24 patients with hepatitis B virus (HBV) infection and 44 healthy controls (HC). Mononuclear cells were separated using a Ficoll gradient, and surface markers were investigated using flow cytometry.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>The frequency of CCR7<jats:sup>\u2212<\/jats:sup>PD\u20101<jats:sup>+<\/jats:sup> Tfh cells was significantly higher in AIH patients (39.1\u2009\u00b1\u20098.6) compared to that in HC (25.1\u2009\u00b1\u20097.9%, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01) and HBV patients (22.7\u2009\u00b1\u20097.8, <jats:italic>P<\/jats:italic>\u2009&lt;\u20090.01). The area under the receiver operating characteristic curve for the frequency of the CCR7<jats:sup>\u2212<\/jats:sup>PD\u20101<jats:sup>+<\/jats:sup> Tfh cell subset for AIH and HC and AIH and HBV was 0.905 and 0.927, respectively. The frequency of the CCR7<jats:sup>\u2212<\/jats:sup>PD\u20101<jats:sup>+<\/jats:sup> Tfh cell subset was not correlated with International Autoimmune Hepatitis Group (IAIHG) scoring, Simplified AIH scoring, or Japanese diagnostic guidelines (R\u2009=\u20090.10, 0.947; R\u2009=\u20090.0008, 0.180; and R\u2009=\u20090.348, 0.558, respectively). Therefore, these frequencies could diagnose AIH patients who were not diagnosed with the IAIHG or simplified AIH scores.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>The frequency of the peripheral CCR7<jats:sup>\u2212<\/jats:sup>PD\u20101<jats:sup>+<\/jats:sup> Tfh cell subset could be useful for diagnosing AIH even in patients who were not diagnosed with IAIHG or simplified AIH scores.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13356","type":"journal-article","created":{"date-parts":[[2019,4,25]],"date-time":"2019-04-25T10:05:07Z","timestamp":1556186707000},"page":"1026-1033","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":6,"title":["Usefulness of chemokine C\u2010C receptor 7<sup>\u2212<\/sup>\/programmed cell death\u20101<sup>+<\/sup> follicular helper T cell subset frequencies in the diagnosis of autoimmune hepatitis"],"prefix":"10.1111","volume":"49","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6683-1219","authenticated-orcid":false,"given":"Naruhiro","family":"Kimura","sequence":"first","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4791-6107","authenticated-orcid":false,"given":"Satoshi","family":"Yamagiwa","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tomoyuki","family":"Sugano","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ryoko","family":"Horigome","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Toru","family":"Setsu","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata Japan"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kentaro","family":"Tominaga","sequence":"additional","affiliation":[{"name":"Division of Gastroenterology and Hepatology Niigata University Graduate School of Medical and Dental Sciences  Niigata 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