{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,5,14]],"date-time":"2025-05-14T01:11:47Z","timestamp":1747185107193,"version":"3.40.5"},"reference-count":25,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2019,12,3]],"date-time":"2019-12-03T00:00:00Z","timestamp":1575331200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","award":["Grant MU 2864\/1\u20103"],"award-info":[{"award-number":["Grant MU 2864\/1\u20103"]}],"id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2020,3]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>Muscarinic acetylcholine receptor type\u00a03\u2010mediated signaling might be involved in the pathogenesis of chronic inflammatory biliary diseases, such as primary biliary cholangitis (PBC) and primary sclerosing cholangitis (PSC). The aim of the present study was to investigate the prevalence of five well\u2010characterized specific single\u2010nucleotide polymorphisms within the muscarinic acetylcholine receptor type\u00a03 gene, <jats:italic>CHRM3<\/jats:italic> (rs11578320, rs6690809, rs6429157, rs7548522, and rs4620530), in patients with PBC and PSC. Patients with chronic hepatitis\u00a0C (CHC) and healthy individuals served as control cohorts. In the PBC cohort, baseline characteristics and response to ursodeoxycholic acid therapy applying established response criteria at 12\u00a0months after the initiation of treatment were evaluated according to the underlying <jats:italic>CHRM3<\/jats:italic> genotype.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p><jats:italic>CHRM3<\/jats:italic> genotyping was carried out in 306 PBC patients, 205 PSC patients, 208 CHC patients, and 240 healthy controls from two independent German tertiary care university centers in Berlin and Leipzig, Germany.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p><jats:italic>CHRM3<\/jats:italic> rs4620530 proportions in patients with PBC significantly differed from patients with PSC (<jats:italic>P<\/jats:italic>\u2009=\u20090.005), CHC (<jats:italic>P<\/jats:italic>\u2009=\u20090.009), and healthy controls (<jats:italic>P<\/jats:italic>\u2009=\u20090.008), primarily due to a substantial overrepresentation of the T\u00a0allele in PBC (49.3% in PBC vs. 39.8% in PSC, 35.7% in CHC, and 40% in healthy controls), indicating a potential association of the rs4620530 T\u00a0allele with PBC (OR 1.461, 95% CI 1.147\u20131.861, <jats:italic>P<\/jats:italic>\u2009=\u20090.002). Further analysis showed no association of <jats:italic>CHRM3<\/jats:italic> single\u2010nucleotide polymorphism rs4620530 with baseline characteristics and ursodeoxycholic acid treatment response in PBC.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusion<\/jats:title><jats:p><jats:italic>CHRM3<\/jats:italic> single\u2010nucleotide polymorphism rs4620530 might confer an increased genetic risk for the development of PBC.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13455","type":"journal-article","created":{"date-parts":[[2019,11,20]],"date-time":"2019-11-20T21:44:21Z","timestamp":1574286261000},"page":"321-329","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Evaluation of muscarinic acetylcholine receptor type\u00a03 gene polymorphisms in patients with primary biliary cholangitis and primary sclerosing cholangitis"],"prefix":"10.1111","volume":"50","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4339-7602","authenticated-orcid":false,"given":"Lena Maria","family":"Greverath","sequence":"first","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Elise","family":"Leicht","sequence":"additional","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nina","family":"Wald de Chamorro","sequence":"additional","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Anne\u2010Christin Beatrice","family":"Wilde","sequence":"additional","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lara Marleen","family":"Steinhagen","sequence":"additional","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Charlotte","family":"Lieb","sequence":"additional","affiliation":[{"name":"Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Moritz","family":"Schmelzle","sequence":"additional","affiliation":[{"name":"Chirurgische Klinik Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sascha","family":"Chopra","sequence":"additional","affiliation":[{"name":"Chirurgische Klinik Charit\u00e9 \u2013 Universit\u00e4tsmedizin Berlin, Campus Virchow Klinikum  Berlin Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oren","family":"Shibolet","sequence":"additional","affiliation":[{"name":"Sackler Faculty of Medicine Tel Aviv University  Tel Aviv Israel"},{"name":"Department of Gastroenterology and Hepatology Tel Aviv\u2010Sourasky Medical Center  Tel Aviv Israel"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janett","family":"Fischer","sequence":"additional","affiliation":[{"name":"Bereich Hepatologie, Klinik 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