{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,1]],"date-time":"2026-10-01T16:11:58Z","timestamp":1790871118916,"version":"4.1.0"},"reference-count":29,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2021,3,6]],"date-time":"2021-03-06T00:00:00Z","timestamp":1614988800000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2021,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Hepatitis B surface antigen (HBsAg) seroclearance indicates a \u201cfunctional cure\u201d in chronic hepatitis B (CHB) virus infection. However, several cases of hepatocellular carcinoma (HCC) development have been reported after HBsAg seroclearance. We evaluated the potential of HBsAg and hepatitis B core\u2010related antigen (HBcrAg), measured by the ultra\u2010highly sensitive assays, in cases with HCC development after HBsAg seroclearance.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled 17 patients with CHB who achieved HBsAg seroclearance, defined by the conventional assay using Architect HBsAg QT kit (five HCC patients and 12 non\u2010HCC patients). HBsAg and HBcrAg were measured in their stored serum samples using ultra\u2010highly sensitive assays featuring \u201cimmunoassay for total antigen including complex via pretreatment (iTACT)\u201d technology.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All five patients who developed HCC were positive for HBsAg or HBcrAg by iTACT\u2010HBsAg or iTACT\u2010HBcrAg at all follow\u2010up points. HBcrAg levels in the HCC group, using iTACT\u2010HBcrAg, were significantly higher than those in the non\u2010HCC group at HBsAg seroclearance (3.6 LogU\/ml (2.8\u20134.2) versus 2.6 (&lt;2.1\u20133.8), <jats:italic>p<\/jats:italic>\u00a0=\u00a00.020). The best cutoff value of iTACT\u2010HBcrAg for predicting HCC development was 2.7 LogU\/ml by receiver operating characteristic curve analysis. The prevalence of HBcrAg \u22652.7 in the HCC group was significantly higher than that in non\u2010HCC group (100% [5\/5] versus 33% [4\/12], <jats:italic>p<\/jats:italic>\u00a0=\u00a00.029).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Residual low viral antigen might predict HCC development even if HBsAg seroclearance was achieved according to a conventional assay. The results suggest that iTACT assays of HBsAg and HBcrAg would be useful for monitoring CHB patients.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13602","type":"journal-article","created":{"date-parts":[[2020,12,3]],"date-time":"2020-12-03T14:37:06Z","timestamp":1607006226000},"page":"426-435","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":37,"title":["Potential of ultra\u2010highly sensitive immunoassays for hepatitis B surface and core\u2010related antigens in patients with or without development of hepatocellular carcinoma after hepatitis B surface antigen seroclearance"],"prefix":"10.1111","volume":"51","author":[{"given":"Fumitaka","family":"Suzuki","sequence":"first","affiliation":[{"name":"Department of Hepatology Toranomon Hospital  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tetsuya","family":"Hosaka","sequence":"additional","affiliation":[{"name":"Department of Hepatology Toranomon Hospital  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Masayasu","family":"Imaizumi","sequence":"additional","affiliation":[{"name":"Product Development Department Fujirebio Inc  Hachioji Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mariko","family":"Kobayashi","sequence":"additional","affiliation":[{"name":"Research Institute for Hepatology Toranomon Hospital  Minato\u2010ku Tokyo Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiharu","family":"Ohue","sequence":"additional","affiliation":[{"name":"Research and Development Department Advanced Life Science Institute, Inc.  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