{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,18]],"date-time":"2026-06-18T15:22:38Z","timestamp":1781796158931,"version":"3.54.5"},"reference-count":39,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2021,6,2]],"date-time":"2021-06-02T00:00:00Z","timestamp":1622592000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["20fk0210045"],"award-info":[{"award-number":["20fk0210045"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100009619","name":"Japan Agency for Medical Research and Development","doi-asserted-by":"publisher","award":["21fk0210094"],"award-info":[{"award-number":["21fk0210094"]}],"id":[{"id":"10.13039\/100009619","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2021,7]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Exercise is effective for the prevention of liver cancer. Exercise exerts biological effects through the regulation of microRNAs (miRNAs) and cytokines\/myokines. We aimed to investigate the effects of low\u2010intensity resistance exercise on serum miRNA and cytokine\/myokine expressions in subjects with no exercise habits.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>We enrolled seven male subjects with no exercise habits in this prospective before\u2010after study. All subjects performed a low\u2010intensity resistance exercise program (three metabolic equivalents, approximately 20\u00a0min\/session). Serum miRNA expressions were evaluated using microarrays. We performed Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis of differentially expressed miRNAs before and after exercise. Serum cytokine\/myokine expressions were evaluated using a multiplex panel.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>All subjects completed the exercise program with no adverse events. In the microarray analysis, seven miRNAs showed a significant change between before and after exercise. Of these, microRNA (miR)\u2010630 and miR\u20105703 showed a &gt;1.5\u2010fold increase (miR\u2010630: 40.7 vs. 69.3 signal intensity, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0133; miR\u20105703: 30.7 vs. 55.9 signal intensity, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0051). KEGG pathway enrichment analysis showed that miR\u2010630\u2010 and miR\u20105703\u2010related genes were enriched in 37 and 5 pathways, including transforming growth factor\u2010beta and Wnt signaling pathways, respectively. In the multiplex analysis, 12 cytokines\/myokines showed significant alteration after exercise compared to before exercise. Of these, fractalkine\/CX3CL1 showed the most significant up\u2010regulation by exercise (94.5 vs. 109.1 pg\/ml, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0017).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>A low\u2010intensity resistance exercise program was associated with upregulation of serum miR\u2010630, miR\u20105703, and fractalkine\/CX3CL1 expressions in subjects with no exercise habits. Thus, even low\u2010intensity exercise may alter miRNA and cytokine\/myokine expressions in humans.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13670","type":"journal-article","created":{"date-parts":[[2021,5,20]],"date-time":"2021-05-20T10:07:00Z","timestamp":1621505220000},"page":"823-833","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":29,"title":["Effects of a low\u2010intensity resistance exercise program on serum miR\u2010630, miR\u20105703, and Fractalkine\/CX3CL1 expressions in subjects with No exercise habits: A preliminary study"],"prefix":"10.1111","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2459-9949","authenticated-orcid":false,"given":"Ryuki","family":"Hashida","sequence":"first","affiliation":[{"name":"Department of Orthopedics School of Medicine Kurume University  Kurume Japan"},{"name":"Division of Rehabilitation Kurume University Hospital  Kurume 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