{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,22]],"date-time":"2026-07-22T18:27:30Z","timestamp":1784744850968,"version":"3.55.0"},"reference-count":44,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2022,2,2]],"date-time":"2022-02-02T00:00:00Z","timestamp":1643760000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2022,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aim<\/jats:title><jats:p>Accurate detection of the hepatic fibrosis stage is essential to estimate the outcome of patients with non\u2010alcoholic fatty liver disease (NAFLD). Many formulas, biomarkers, and imaging tests are being developed to predict advanced liver fibrosis without performing a liver biopsy. However, these tests do not have high efficiency in detecting early\u2010stage hepatic fibrosis. Therefore, we aimed to detect the presence of hepatic fibrosis (\u2265F1) merely by using only standard clinical markers.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>A total of 436 patients with NAFLD who underwent liver biopsy were retrospectively enrolled as the discovery cohort (316 patients) and the validation cohort (120 patients). Liver biopsy and laboratory data were matched to extract simple parameters for identifying \u2265F1.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>We developed a novel simplified \u2265F1 detecting system, designated as 2\u2010Step PLT16\u2010AST44 method, where (1) PLT of 16\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\/\u03bcl or less, or (2) PLT greater than 16\u00a0\u00d7\u00a010<jats:sup>4<\/jats:sup>\/\u03bcl and AST greater than 44\u00a0U\/L is determined as having \u2265F1 fibrosis. The 2\u2010Step PLT16\u2010AST44 method had a sensitivity of 68%, a specificity of 90%, a positive predictive value (PPV) of 97%, a negative predictive value (NPV) of 40%, and an accuracy of 72% to detect \u2265F1 fibrosis in the discovery cohort. Validation studies further supported these results. Despite its simplicity, the 2\u2010Step PLT16\u2010AST44 method's power to detect \u2265F1 fibrosis in total NAFLD patients was comparable to hyaluronic acid, type 4 collagen 7S, FIB\u20104, and APRI.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We propose the 2\u2010Step PLT16\u2010AST44 method as a simple and beneficial early\u2010stage hepatic fibrosis detection system.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13745","type":"journal-article","created":{"date-parts":[[2022,1,18]],"date-time":"2022-01-18T13:00:22Z","timestamp":1642510822000},"page":"352-363","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":14,"title":["2\u2010Step PLT16\u2010AST44 method: Simplified liver fibrosis detection system in patients with non\u2010alcoholic fatty liver disease"],"prefix":"10.1111","volume":"52","author":[{"given":"Naoyuki","family":"Fujimori","sequence":"first","affiliation":[{"name":"Department of 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