{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,27]],"date-time":"2026-08-27T10:51:39Z","timestamp":1787827899514,"version":"build-2784847793"},"reference-count":53,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2023,11,22]],"date-time":"2023-11-22T00:00:00Z","timestamp":1700611200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:sec><jats:title>Aims<\/jats:title><jats:p>Biliary atresia (BA) is characterized by intrahepatic inflammation and rapid progression of liver fibrosis. Galectin\u20103, a beta\u2010galactoside binding protein, is a key regulator of inflammation and fibrosis. The aim of this study was to characterize circulating and hepatic Galectin\u20103 levels in children with BA.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods<\/jats:title><jats:p>Plasma and liver samples were obtained from children with early BA at time of Kasai hepatoportoenterostomy, late BA at time of transplant, early and late other cholestatic liver diseases (CLD), and controls. Plasma Galectin\u20103 was measured using standard enzyme\u2010linked immunoassay. Liver tissue was analyzed with multiplex immunohistochemistry and quantified using whole slide analysis. Statistical comparisons were made using nonparametric testing.<\/jats:p><\/jats:sec><jats:sec><jats:title>Results<\/jats:title><jats:p>Plasma Galectin\u20103 in late BA was significantly higher than in early BA (20.82 [12.45\u201330.46] vs. 11.30 [8.74\u201316.83] ng\/mL, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.0096). Galectin\u20103 levels correlated with markers of disease severity and interleukin\u20106. There were significantly more Galectin\u20103<jats:sup>+<\/jats:sup> M2 macrophages in late BA in comparison to late other CLD (162 [157\u2013233] vs. 49 [33\u201359] cells\/mm<jats:sup>2<\/jats:sup>, <jats:italic>p<\/jats:italic>\u00a0=\u00a00.03). The number of Galectin\u20103<jats:sup>+<\/jats:sup> M2 macrophages correlated with the number of activated hepatic stellate cells and bile duct proliferation.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Plasma Galectin\u20103 is higher in late BA at time of transplant in comparison to early BA at time of Kasai. The number of Galectin\u20103 expressing M2 macrophages in late BA is elevated relative to late other CLD and was associated with other prognostic histological findings. Galectin\u20103 targeted therapy may be beneficial in slowing disease progression to cirrhosis in children with BA.<\/jats:p><\/jats:sec>","DOI":"10.1111\/hepr.13987","type":"journal-article","created":{"date-parts":[[2023,11,11]],"date-time":"2023-11-11T09:10:37Z","timestamp":1699693837000},"page":"392-402","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Galectin\u20103 in biliary atresia and other pediatric cholestatic liver diseases"],"prefix":"10.1111","volume":"54","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6993-255X","authenticated-orcid":false,"given":"Dor","family":"Yoeli","sequence":"first","affiliation":[{"name":"Division of Transplant Surgery Department of Surgery University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cara L.","family":"Mack","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition Department of Pediatrics Children's Hospital Colorado University of Colorado Anschutz Medical Campus  Aurora Colorado USA"},{"name":"Division of Pediatric Gastroenterology, Hepatology and Nutrition Department of Pediatrics Children's Wisconsin Medical College of Wisconsin  Milwaukee Wisconsin USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yuhuan","family":"Luo","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition Department of Pediatrics Children's Hospital Colorado University of Colorado Anschutz Medical Campus  Aurora Colorado USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexander","family":"Chaidez","sequence":"additional","affiliation":[{"name":"Section of Pediatric Gastroenterology, Hepatology and Nutrition Department of Pediatrics Children's Hospital Colorado University of Colorado Anschutz Medical Campus  Aurora Colorado 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