{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,21]],"date-time":"2026-10-21T14:17:44Z","timestamp":1792592264964,"version":"4.5.0"},"reference-count":40,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T00:00:00Z","timestamp":1774224000000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2026,7]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Aim<\/jats:title>\n                    <jats:p>To identify autoimmune hepatitis (AIH)\u2010specific autoantibodies using a high\u2010content human protein microarray and evaluate their diagnostic performance.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>\n                      Pretreatment sera from untreated patients with AIH (\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a068), primary biliary cholangitis (PBC,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a019), metabolic dysfunction\u2010associated steatotic liver disease (MASLD,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a039), chronic hepatitis C (CHC,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a027), and drug\u2010induced liver injury (DILI,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a018) and healthy controls (HC,\n                      <jats:italic>n<\/jats:italic>\n                      \u00a0=\u00a037) were screened with a wheat\u2010germ, cell\u2010free microarray displaying &gt;\u00a016,000 proteins. Candidate antigens were validated by ELISA, and antibody titers were correlated with clinical variables, treatment response, and outcomes.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      Mitochondrial ribosomal protein S27 (MRPS27) emerged as the dominant AIH\u2010associated antigen. The mean anti\u2010MRPS27 antibody titer in untreated patients with AIH (50.2 AU) exceeded that in patients with PBC (9.3 AU), MASLD (12.8 AU), CHC (24.8 AU), DILI (19.8 AU), and HC (7.5 AU, all\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). A cutoff of 35.4 AU (HC mean\u00a0+\u00a02 SD) yielded a sensitivity of 61.8%, a specificity of 87.1%, a positive predictive value of 70.0%, and a negative predictive value of 82.4%. Positivity was unrelated to baseline histology, biochemistry or IgG, and titers were unchanged after corticosteroid induction. Interestingly, patients with low titers experienced more liver\u2010related events during follow\u2010up.\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>The anti\u2010MRPS27 antibody is a promising adjunctive serological marker that enhances the specificity of AIH diagnosis. The pathogenic significance and prognostic value of this antibody warrant prospective validation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hepr.70169","type":"journal-article","created":{"date-parts":[[2026,3,23]],"date-time":"2026-03-23T17:39:47Z","timestamp":1774287587000},"page":"1127-1139","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":4,"title":["Anti\u2010MRPS27 Antibody: A Novel Diagnostic Biomarker for Autoimmune Hepatitis Identified via a Human Proteome Microarray"],"prefix":"10.1111","volume":"56","author":[{"given":"Jun","family":"Wada","sequence":"first","affiliation":[{"name":"Department of Gastroenterology Fukushima Medical University School of Medicine  Fukushima Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5359-9465","authenticated-orcid":false,"given":"Kazumichi","family":"Abe","sequence":"additional","affiliation":[{"name":"Department 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