{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T11:58:06Z","timestamp":1791201486679,"version":"4.2.0"},"reference-count":41,"publisher":"Wiley","issue":"10","license":[{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"},{"start":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T00:00:00Z","timestamp":1781827200000},"content-version":"tdm","delay-in-days":0,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1.1"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81902156"],"award-info":[{"award-number":["81902156"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Hepatology Research"],"published-print":{"date-parts":[[2026,10]]},"abstract":"<jats:title>ABSTRACT<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>The early detection of hepatocellular carcinoma (HCC) remains a significant challenge. This study aimed to develop a quantitative PCR (qPCR) assay to analyze the fragment size distribution of cfDNA at the enhancer region of the oncogene MYC, evaluate its potential ability as a diagnostic biomarker for HCC.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>In this retrospective case\u2010control study, we enrolled 40 patients with newly diagnosed HCC, 45 patients with cirrhosis, and 130 healthy volunteers. We designed two overlapping primer pairs to amplify a long (432 bp) and a short (65 bp) fragment via qPCR targeting MYC enhancer region. The Fragment Index (FI\u2010myc), defined as the ratio of short to long fragment, was calculated to infer regional chromatin accessibility, and the diagnostic performance of FI\u2010myc was evaluated using receiver operating characteristic (ROC) curve and logistic regression analyses.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>\n                      FI\u2010myc levels were significantly elevated in HCC patients compared to those with cirrhosis and healthy controls (\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.01). Using the cutoff of 1.87, FI\u2010myc demonstrated a sensitivity of 85.02% for discriminating HCC from cirrhosis and healthy controls. The area under the ROC curve (AUC) for FI\u2010myc was 0.81, which was significantly superior to that of alpha\u2010fetoprotein (AFP) (AUC\u00a0=\u00a00.49,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.001). FI\u2010myc maintained high sensitivity of 80.09% in early\u2010stage HCC, and could detect 81.49% of HCC patients with negative AFP. Multivariate analysis demonstrated FI\u2010myc as an independent indicator for HCC (OR\u00a0=\u00a02.82,\n                      <jats:italic>p<\/jats:italic>\n                      \u00a0&lt;\u00a00.05).\n                    <\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusion<\/jats:title>\n                    <jats:p>We developed a qPCR\u2010based assay to infer MYC enhancer accessibility. The FI\u2010myc index represents a promising liquid biopsy biomarker for early HCC detection, particularly as a complementary tool in AFP\u2010negative cases, showing potential for clinical translation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1111\/hepr.70222","type":"journal-article","created":{"date-parts":[[2026,6,19]],"date-time":"2026-06-19T09:54:43Z","timestamp":1781862883000},"page":"1516-1523","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Fragmentation Analysis of the MYC Enhancer in Cell\u2010Free DNA by qPCR for Early Detection of Hepatocellular Carcinoma"],"prefix":"10.1111","volume":"56","author":[{"given":"Yan","family":"Jiang","sequence":"first","affiliation":[{"name":"Department of Laboratory Medicine The Second Affiliated Hospital of Zhejiang University  Hangzhou 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Province Affiliated to Zhejiang Chinese Medical University (College of Integrated Traditional Chinese and Western Medicine Clinical Medicine)  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Depeng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Laboratory Medicine The Second Affiliated Hospital of Zhejiang University  Hangzhou China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tao","family":"Chen","sequence":"additional","affiliation":[{"name":"Department of Blood Transfusion Zhejiang Hospital  Hangzhou 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