{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,9]],"date-time":"2026-06-09T20:42:10Z","timestamp":1781037730752,"version":"3.54.1"},"reference-count":10,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2012,12,21]],"date-time":"2012-12-21T00:00:00Z","timestamp":1356048000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2013,2]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>Superparamagnetic iron oxide nanoparticle (<jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>)\u2010enhanced axillary lymph node magnetic resonance imaging (<jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content>) has the potential to supersede sentinel lymph node (<jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>) biopsy in the management of early breast cancer with <jats:italic>in vivo<\/jats:italic> pre\u2010operative metastasis detection. We evaluated the distribution and extent of <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s within the <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> and its impact on routine histological assessment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>A total of 131 <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>s from 51 consecutive patients with breast cancer were examined histologically following subcutaneous injection of 2\u20134\u00a0ml <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s. <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content>s were identified intra\u2010operatively using the combined technique. <jats:italic>Ex vivo<\/jats:italic> histological analysis was performed and the distribution of <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s assessed using standard haematoxylin and eosin staining. <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content> distribution was predominantly within lymph node sinuses (81%); fewer nodes contained iron in the subcapsular space (24%) and parenchyma (14%). In 58%, <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s were sequestered in macrophages. <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s were not seen within the areas of nodes containing metastases, but was seen surrounding the metastases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>Following subcutaneous injection of <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>s, iron deposition is found predominantly in sinuses and the subcapsular space and is not found in areas containing metastases. This reinforces the notion that high resolution <jats:styled-content style=\"fixed-case\">SPIO<\/jats:styled-content>\u2010enhanced <jats:styled-content style=\"fixed-case\">MRI<\/jats:styled-content> has the potential for <jats:italic>in vivo<\/jats:italic> metastasis in the <jats:styled-content style=\"fixed-case\">SLN<\/jats:styled-content> in breast cancer. Routine histological examination was unaffected.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12019","type":"journal-article","created":{"date-parts":[[2012,8,24]],"date-time":"2012-08-24T04:19:09Z","timestamp":1345781949000},"page":"481-486","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":65,"title":["Deposition of superparamagnetic iron\u2010oxide nanoparticles in axillary sentinel lymph nodes following subcutaneous injection"],"prefix":"10.1111","volume":"62","author":[{"given":"Laura","family":"Johnson","sequence":"first","affiliation":[{"name":"Department of Research Oncology Kings College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sarah E","family":"Pinder","sequence":"additional","affiliation":[{"name":"Department of Research Oncology Kings College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Douek","sequence":"additional","affiliation":[{"name":"Department of Research Oncology Kings College London London UK"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2012,12,21]]},"reference":[{"key":"e_1_2_8_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejso.2009.03.008"},{"key":"e_1_2_8_3_1","first-page":"315","article-title":"Use of axillary ultrasonography in breast cancer: a useful tool to reduce sentinel node procedures","volume":"80","author":"Sianesi M","year":"2009","journal-title":"Ann. Ital. Chir."},{"key":"e_1_2_8_4_1","doi-asserted-by":"publisher","DOI":"10.1093\/jnci\/djj158"},{"key":"e_1_2_8_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.addr.2006.09.013"},{"key":"e_1_2_8_6_1","doi-asserted-by":"publisher","DOI":"10.1038\/nrc1566"},{"key":"e_1_2_8_7_1","article-title":"Differentiation of reactive and tumor metastatic lymph nodes with diffusion\u2010weighted and spio\u2010enhanced MRI","author":"Zhang F","year":"2012","journal-title":"Mol. Imag. Biol."},{"key":"e_1_2_8_8_1","doi-asserted-by":"publisher","DOI":"10.1056\/NEJMoa022749"},{"key":"e_1_2_8_9_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pmed.0010066"},{"key":"e_1_2_8_10_1","doi-asserted-by":"publisher","DOI":"10.1245\/s10434-011-1710-7"},{"key":"e_1_2_8_11_1","doi-asserted-by":"publisher","DOI":"10.1002\/ana.410320713"}],"container-title":["Histopathology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.1111%2Fhis.12019","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.1111\/his.12019","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2023,10,15]],"date-time":"2023-10-15T16:28:31Z","timestamp":1697387311000},"score":1,"resource":{"primary":{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/10.1111\/his.12019"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2012,12,21]]},"references-count":10,"journal-issue":{"issue":"3","published-print":{"date-parts":[[2013,2]]}},"alternative-id":["10.1111\/his.12019"],"URL":"https:\/\/doi.org\/10.1111\/his.12019","archive":["Portico"],"relation":{},"ISSN":["0309-0167","1365-2559"],"issn-type":[{"value":"0309-0167","type":"print"},{"value":"1365-2559","type":"electronic"}],"subject":[],"published":{"date-parts":[[2012,12,21]]},"assertion":[{"value":"2012-08-20","order":1,"name":"accepted","label":"Accepted","group":{"name":"publication_history","label":"Publication History"}},{"value":"2012-12-21","order":2,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}