{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,18]],"date-time":"2026-10-18T01:23:58Z","timestamp":1792286638904,"version":"4.4.0"},"reference-count":52,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2013,4,9]],"date-time":"2013-04-09T00:00:00Z","timestamp":1365465600000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2013,6]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>To investigate in prion diseases the <jats:italic>in\u2010situ<\/jats:italic> localization of prion protein oligomers sharing a common epitope with amyloid oligomers involved in a range of neurodegenerative diseases.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>We performed immunohistochemistry on sporadic Creutzfeldt\u2013Jakob disease (sCJD) (<jats:italic>n<\/jats:italic>\u00a0=\u00a09) and hereditary Gerstmann\u2013Str\u00e4ussler\u2013Scheinker disease (GSS) (<jats:italic>n<\/jats:italic>\u00a0=\u00a01) specimens with the anti\u2010oligomer antibody A11 to determine the localization of reactive species. We found that A11 reactivity in the sCJD specimens was localized to the cerebral and cerebellar cortices both in spongiform and adjacent, non\u2010spongiform areas, reminiscent of multicentric or diffuse plaques. In the GSS specimens, we found that staining was closely associated with kuru\u2010like plaques, and that A11\u2010reactive species colocalized with protease\u2010resistant prion protein (Prp<jats:sup>Sc<\/jats:sup>). We also observed sporadic neuronal cytosolic staining in both types of specimen.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We confirm that intracellular and extracellular A11\u2010reactive species are present <jats:italic>in\u00a0situ<\/jats:italic> in sCJD cases and GSS, and that immunoreactivity for A11 and Prp<jats:sup>Sc<\/jats:sup> overlaps. We argue that the A11\u2010reactive species are indeed composed of oligomeric Prp<jats:sup>Sc<\/jats:sup>, and suggest that the toxic effects of Prp<jats:sup>Sc<\/jats:sup> oligomers could be related to the generic oligomeric conformation recognized by A11.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12097","type":"journal-article","created":{"date-parts":[[2013,1,18]],"date-time":"2013-01-18T07:06:50Z","timestamp":1358492810000},"page":"994-1001","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":10,"title":["Localization of A11\u2010reactive oligomeric species in prion diseases"],"prefix":"10.1111","volume":"62","author":[{"given":"Frederik H","family":"Aidt","sequence":"first","affiliation":[{"name":"Section of Molecular Medicine Department of Clinical Biochemistry, Immunology and Genetics Statens Serum Institut Copenhagen Denmark"},{"name":"Institute of Cellular and Molecular Medicine Faculty of Health Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lis F","family":"Hasholt","sequence":"additional","affiliation":[{"name":"Institute of Cellular and Molecular Medicine Faculty of Health Sciences University of Copenhagen Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Michael","family":"Christiansen","sequence":"additional","affiliation":[{"name":"Section of Molecular Medicine Department of Clinical Biochemistry, Immunology and Genetics Statens Serum Institut Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henning","family":"Laursen","sequence":"additional","affiliation":[{"name":"Neuropathology Laboratory Rigshospitalet Copenhagen Denmark"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","published-online":{"date-parts":[[2013,4,9]]},"reference":[{"key":"e_1_2_5_2_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.6801762"},{"key":"e_1_2_5_3_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.23.10962"},{"key":"e_1_2_5_4_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1750-3639.1995.tb00625.x"},{"key":"e_1_2_5_5_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1073619"},{"key":"e_1_2_5_6_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0968-0004(01)01792-3"},{"key":"e_1_2_5_7_1","doi-asserted-by":"publisher","DOI":"10.1093\/emboj\/cdf653"},{"key":"e_1_2_5_8_1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.26.15137"},{"key":"e_1_2_5_9_1","volume-title":"Prion biology and disease","author":"Prusiner SB","year":"2004"},{"key":"e_1_2_5_10_1","doi-asserted-by":"publisher","DOI":"10.1093\/brain\/104.3.535"},{"key":"e_1_2_5_11_1","doi-asserted-by":"publisher","DOI":"10.1093\/bmb\/66.1.255"},{"key":"e_1_2_5_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1742-4658.2010.07721.x"},{"key":"e_1_2_5_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/s12017-007-0003-6"},{"key":"e_1_2_5_14_1","doi-asserted-by":"publisher","DOI":"10.1126\/science.1079469"},{"key":"e_1_2_5_15_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.C400260200"},{"key":"e_1_2_5_16_1","doi-asserted-by":"publisher","DOI":"10.1038\/nn.3120"},{"key":"e_1_2_5_17_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.neuro.26.010302.081142"},{"key":"e_1_2_5_18_1","doi-asserted-by":"publisher","DOI":"10.1002\/jcb.22743"},{"key":"e_1_2_5_19_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbrc.2004.12.172"},{"key":"e_1_2_5_20_1","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.0030125"},{"key":"e_1_2_5_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0092-8674(00)81572-X"},{"key":"e_1_2_5_22_1","doi-asserted-by":"publisher","DOI":"10.1002\/path.2576"},{"key":"e_1_2_5_23_1","doi-asserted-by":"publisher","DOI":"10.1038\/emboj.2011.86"},{"key":"e_1_2_5_24_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2007.04.001"},{"key":"e_1_2_5_25_1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.2807\/ese.17.15.20142-en","article-title":"Description and analysis of 12\u00a0years of surveillance for Creutzfeldt\u2013Jakob disease in Denmark, 1997 to 2008","volume":"17","author":"Gubbels S","year":"2012","journal-title":"Euro. 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