{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,10]],"date-time":"2026-09-10T03:14:00Z","timestamp":1789010040950,"version":"build-2803163510"},"reference-count":28,"publisher":"Wiley","issue":"4","license":[{"start":{"date-parts":[[2013,8,8]],"date-time":"2013-08-08T00:00:00Z","timestamp":1375920000000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2013,10]]},"abstract":"<jats:sec><jats:title>Aim<\/jats:title><jats:p>To assess the validity and potential clinical utility of evaluating <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> expression by immunohistochemistry (<jats:styled-content style=\"fixed-case\">IHC<\/jats:styled-content>) in mantle cell lymphoma (<jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>).<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">MYC IHC<\/jats:styled-content> was scored on a tissue microarray containing 62 <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>s and 29 controls by two pathologists. Inter\u2010observer correlation was high (intra\u2010class correlation of 0.98). <jats:styled-content style=\"fixed-case\">MYC IHC<\/jats:styled-content> scores correlated with <jats:italic><jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content><\/jats:italic> expression (Spearman's rank correlation 0.69, <jats:italic>P<\/jats:italic>\u00a0<jats:italic>&lt;\u00a0<\/jats:italic>0.0001) and weakly with Ki67 proliferation index (Spearman's rank correlation 0.30, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.03). Six blastic MCLs did not have higher mean <jats:styled-content style=\"fixed-case\">MYC IHC<\/jats:styled-content> scores or <jats:italic><jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content><\/jats:italic> <jats:styled-content style=\"fixed-case\">mRNA<\/jats:styled-content> expression than non\u2010blastic <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>s. None of 57 cases assessed, including all of the blastic cases, showed <jats:italic><jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content><\/jats:italic> rearrangement by fluorescence <jats:italic>in\u2010situ<\/jats:italic> hybridization. Multivariate analysis with backward selection from potential predictors including age, lactate dehydrogenase, leukocyte count, <jats:styled-content style=\"fixed-case\">MIPI<\/jats:styled-content> score, <jats:styled-content style=\"fixed-case\">ECOG<\/jats:styled-content> performance status, blastic morphology and Ki67 index showed that <jats:styled-content style=\"fixed-case\">MYC IHC<\/jats:styled-content> score is an independent predictor of progression\u2010free survival (hazard ratio 2.34, 95% CI 1.42\u20133.88, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.0009) and overall survival (hazard ratio 1.90, 95% CI 1.05\u20133.43, <jats:italic>P<\/jats:italic>\u00a0=\u00a00.034).<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p>We show that a new monoclonal anti\u2010<jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> antibody can enable accurate and reproducible visual assessment of <jats:styled-content style=\"fixed-case\">MYC<\/jats:styled-content> expression that is independently predictive of clinical outcomes in <jats:styled-content style=\"fixed-case\">MCL<\/jats:styled-content>.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12207","type":"journal-article","created":{"date-parts":[[2013,6,17]],"date-time":"2013-06-17T08:22:38Z","timestamp":1371457358000},"page":"499-508","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":28,"title":["Immunohistochemical evaluation of <scp>MYC<\/scp> expression in mantle cell lymphoma"],"prefix":"10.1111","volume":"63","author":[{"given":"Matthew J","family":"Oberley","sequence":"first","affiliation":[{"name":"Department of Pathology and Laboratory Medicine University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Saurabh A","family":"Rajguru","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chong","family":"Zhang","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Medical Informatics University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"KyungMann","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biostatistics and Medical Informatics University of Wisconsin Madison WI USA"},{"name":"University of Wisconsin Carbone Cancer Center University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gene R","family":"Shaw","sequence":"additional","affiliation":[{"name":"Department of Pathology Marshfield Clinic Marshfield WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kreg M","family":"Grindle","sequence":"additional","affiliation":[{"name":"University of Wisconsin Carbone Cancer Center University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Brad S","family":"Kahl","sequence":"additional","affiliation":[{"name":"Department of Medicine University of Wisconsin Madison WI USA"},{"name":"University of Wisconsin Carbone Cancer Center University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig","family":"Kanugh","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jennifer","family":"Laffin","sequence":"additional","affiliation":[{"name":"Wisconsin State Laboratory of Hygiene University of Wisconsin Madison WI USA"},{"name":"Department of Pediatrics University of Wisconsin Madison WI USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"David T","family":"Yang","sequence":"additional","affiliation":[{"name":"Department of Pathology and Laboratory Medicine 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