{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T04:41:16Z","timestamp":1774932076730,"version":"3.50.1"},"reference-count":22,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2013,8,29]],"date-time":"2013-08-29T00:00:00Z","timestamp":1377734400000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2013,11]]},"abstract":"<jats:sec><jats:title>Aims<\/jats:title><jats:p>The aim of this study was to assess protein and m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression of epithelial membrane protein 1 (<jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1) in the nasal mucosa of patients with nasal polyps (<jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>), and to determine what changes occur in response to glucocorticosteroid (<jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>) treatment.<\/jats:p><\/jats:sec><jats:sec><jats:title>Methods and results<\/jats:title><jats:p>NP tissue was obtained from 55 patients, 18 of whom were treated with nasal <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content>s (i.e. these 18 patients had <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> biopsies taken before and after treatment). Biopsies of inferior turbinate mucosa from 30 healthy subjects were used as controls. Quantitative <jats:styled-content style=\"fixed-case\">PCR<\/jats:styled-content> and immunohistochemistry were performed to determine the expression levels of <jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1. <jats:italic><jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> expression was significantly lower (2.77\u2010fold) in tissues from <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients before <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> treatment when compared to controls, but was increased in these patients after <jats:styled-content style=\"fixed-case\">GC<\/jats:styled-content> treatment. <jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1 staining in nasal epithelium co\u2010localized with both basal (p63<jats:sup>+<\/jats:sup>) and differentiated (<jats:styled-content style=\"fixed-case\">CK<\/jats:styled-content>18<jats:sup>+<\/jats:sup>) epithelial cells. Their immunoreactivity was significantly greater in controls than <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content> patients. <jats:italic><jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1<\/jats:italic> m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content> levels were lower in the epithelium with severe hyperplasia (1.79\u2010fold) or with metaplasia (1.85\u2010fold) as compared to those with mild to moderate hyperplasia or non\u2010metaplastic epithelium, respectively. Positive correlations between <jats:italic><jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1<\/jats:italic> and other epithelial cell\u2010related gene (e.g. <jats:italic><jats:styled-content style=\"fixed-case\">JUN<\/jats:styled-content>,<jats:styled-content style=\"fixed-case\"> PTGS<\/jats:styled-content>2, <jats:styled-content style=\"fixed-case\">AREG<\/jats:styled-content><\/jats:italic> etc.) m<jats:styled-content style=\"fixed-case\">RNA<\/jats:styled-content>s were observed.<\/jats:p><\/jats:sec><jats:sec><jats:title>Conclusions<\/jats:title><jats:p><jats:styled-content style=\"fixed-case\">EMP<\/jats:styled-content>1 could be a biomarker for aberrant epithelial remodelling and metaplasia in chronic inflammatory upper airway mucosa (e.g. <jats:styled-content style=\"fixed-case\">NP<\/jats:styled-content>).<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12211","type":"journal-article","created":{"date-parts":[[2013,6,21]],"date-time":"2013-06-21T04:56:02Z","timestamp":1371790562000},"page":"686-695","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":26,"title":["Down\u2010regulation of <scp>EMP<\/scp>1 is associated with epithelial hyperplasia and metaplasia in nasal polyps"],"prefix":"10.1111","volume":"63","author":[{"given":"Xue Min","family":"Yu","sequence":"first","affiliation":[{"name":"Department of Otolaryngology Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chun Wei","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore National University Health System Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ying Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore National University Health System Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jing","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore National University Health System Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhi Bin","family":"Lin","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tian Ying","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology The First Affiliated Hospital of Sun Yat\u2010Sen University Guangzhou China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Zhao","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xin Liang","family":"Pan","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Li","family":"Shi","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology Qilu Hospital Shandong University Jinan China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"De Yun","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Otolaryngology National University of Singapore National University Health System Singapore City Singapore"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2013,8,29]]},"reference":[{"key":"e_1_2_7_2_1","first-page":"1","article-title":"Epos 2012: European position paper on rhinosinusitis and nasal polyps 2012. 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