{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:16:53Z","timestamp":1790749013880,"version":"4.1.0"},"reference-count":35,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2013,9,20]],"date-time":"2013-09-20T00:00:00Z","timestamp":1379635200000},"content-version":"vor","delay-in-days":0,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Histopathology"],"published-print":{"date-parts":[[2013,12]]},"abstract":"Aims<\/jats:title>CD<\/jats:styled-content>30 expression by bone marrow (BM<\/jats:styled-content>) mast cells (MC<\/jats:styled-content>) has been reported recently in systemic mastocytosis (SM<\/jats:styled-content>) patients. The aim of this study was to investigate the potential diagnostic and prognostic value of CD<\/jats:styled-content>30 expression in SM<\/jats:styled-content> as assessed by multiparameter flow cytometry.<\/jats:p><\/jats:sec>Methods and results<\/jats:title>A total of 163 consecutive BM<\/jats:styled-content> samples corresponding to 142 SM<\/jats:styled-content> patients and 21 non\u2010mastocytosis cases were studied. CD<\/jats:styled-content>30 was positive in most SM<\/jats:styled-content> patients (80%), but in only one non\u2010mastocytosis case (4.8%). When combined with CD<\/jats:styled-content>25, CD<\/jats:styled-content>30 contributed to an improved accuracy over that of CD<\/jats:styled-content>25 alone (98% versus 93%) mainly because most (eight of nine) of the well\u2010differentiated SM<\/jats:styled-content> (WDSM<\/jats:styled-content>), who lacked CD<\/jats:styled-content>25, were CD<\/jats:styled-content>30+<\/jats:sup>. Similar levels of expression of CD<\/jats:styled-content>30 were observed among all different subgroups of SM<\/jats:styled-content> except mast cell leukaemia; among indolent SM<\/jats:styled-content> (ISM<\/jats:styled-content>) patients, no significant association was observed between the levels of CD<\/jats:styled-content>30 expression and other clinical and biological features of the disease.<\/jats:p><\/jats:sec>Conclusions<\/jats:title>The increased expression of CD<\/jats:styled-content>30 associated with absence of CD<\/jats:styled-content>25 contributes to the diagnosis of WDSM<\/jats:styled-content> and its distinction from other subtypes of SM<\/jats:styled-content>. By contrast, CD<\/jats:styled-content>30 expression did not contribute either to prognostic stratification of ISM<\/jats:styled-content> or to the differential diagnosis between ISM<\/jats:styled-content> and aggressive SM<\/jats:styled-content> cases.<\/jats:p><\/jats:sec>","DOI":"10.1111\/his.12221","type":"journal-article","created":{"date-parts":[[2013,7,4]],"date-time":"2013-07-04T10:24:08Z","timestamp":1372933448000},"page":"780-787","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":95,"title":["CD<\/scp>30 expression by bone marrow mast cells from different diagnostic variants of systemic mastocytosis"],"prefix":"10.1111","volume":"63","author":[{"given":"Jos\u00e9 M","family":"Morgado","sequence":"first","affiliation":[{"name":"Instituto de Estudios de Mastocitosis de Castilla La Mancha Toledo Spain"},{"name":"Red Espa\u00f1ola de Mastocitosis Toledo Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Omar","family":"Perbellini","sequence":"additional","affiliation":[{"name":"Section of Haematology Azienda Ospedaliera Universitaria Integrata and University of Verona Verona Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ryan C","family":"Johnson","sequence":"additional","affiliation":[{"name":"Department of Pathology School of Medicine Stanford University Stanford CA USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina","family":"Teod\u00f3sio","sequence":"additional","affiliation":[{"name":"Red Espa\u00f1ola de Mastocitosis Toledo Spain"},{"name":"Servicio General de Citometr\u00eda, Centro de Investigaci\u00f3n del C\u00e1ncer (IBMCC\u2010CSIC\/USAL; 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